Phospho-MARCKS (Ser159) Antibody - #AF3250

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产品: 磷酸化 MARCKS (Ser159) 抗体
货号: AF3250
描述: Rabbit polyclonal antibody to Phospho-MARCKS (Ser159)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Chicken, Xenopus
分子量: 36,80kDa; 32kD(Calculated).
蛋白号: P29966
RRID: AB_2834676

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 50ul RMB¥ 1300 现货
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 200ul RMB¥ 3200 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-MARCKS (Ser159) Antibody detects endogenous levels of MARCKS only when phosphorylated at Serine 159.
RRID:
AB_2834676
引用格式: Affinity Biosciences Cat# AF3250, RRID:AB_2834676.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate;

抗原和靶标

免疫原:
Uniprot:

P29966(MARCS_HUMAN) >>Visit HPA database.

基因/基因ID:
MARCKS(4082)
描述:
MARCKS the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development.
序列:
MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAPAADKEEPAAAGSGAASPSAAEKGEPAAAAAPEAGASPVEKEAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGAAAAAAEAGAASGEQAAAPGEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASAAACEAPSAAGPGAPPEQEAAPAEEPAAAAASSACAAPSQEAQPECSPEAPPAEAAE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29966 作为底物

Site PTM Type Enzyme
G2 Myristoylation
K11 Ubiquitination
S26 Phosphorylation P24941 (CDK2)
S27 Phosphorylation P24941 (CDK2) , P17252 (PRKCA)
S29 Phosphorylation
K30 Ubiquitination
S46 Phosphorylation P17252 (PRKCA)
S52 Phosphorylation
K55 Ubiquitination
S63 Phosphorylation
K69 Ubiquitination
S77 Phosphorylation
S81 Phosphorylation P17252 (PRKCA)
S83 Phosphorylation
K87 Ubiquitination
S101 Phosphorylation P17252 (PRKCA)
K105 Ubiquitination
S118 Phosphorylation P17252 (PRKCA) , P24941 (CDK2)
T120 Phosphorylation
S128 Phosphorylation
S131 Phosphorylation P24941 (CDK2)
S132 Phosphorylation P24941 (CDK2)
T133 Phosphorylation P24941 (CDK2)
S134 Phosphorylation P24941 (CDK2)
S135 Phosphorylation P24941 (CDK2)
T143 Phosphorylation
S145 Phosphorylation
S147 Phosphorylation P24941 (CDK2)
T150 Phosphorylation P24941 (CDK2)
K152 Ubiquitination
K153 Ubiquitination
S159 Phosphorylation Q13464 (ROCK1) , P17252 (PRKCA) , P05771 (PRKCB) , P17612 (PRKACA) , Q16512 (PKN1)
K161 Methylation
S163 Phosphorylation P17252 (PRKCA) , Q16512 (PKN1) , P05771 (PRKCB)
K165 Ubiquitination
S167 Phosphorylation P17252 (PRKCA)
S170 Phosphorylation P17252 (PRKCA) , Q16512 (PKN1) , P05771 (PRKCB)
K172 Acetylation
K172 Methylation
K176 Ubiquitination
K248 Methylation
S252 Phosphorylation
S262 Phosphorylation
K267 Ubiquitination
S314 Phosphorylation

研究背景

功能:

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

翻译修饰:

Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

细胞定位:

Cytoplasm>Cytoskeleton. Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the MARCKS family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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