SETD2 Antibody - #AF7552

Figure 3. Interference with TFAP2C disrupted gene expression patterns. (A) The volcano shows differentially expressed genes between control group and TFAP2C-KD group (six to eight IVF embryos at four-cell and eight-cell stages in each group). Each dot represents a gene; the red dots indicate the upregulated gene and the blue dots indicate the downregulated genes; false discovery rate < 0.1, |log2FC| 1.5; (B) pathway enrichment of upregulated genes and downregulated genes; (C) heatmap of main epigenetic genes in control group and TFAP2C-KD group from RNA sequencing data (each sample includes six to eight IVF embryos at four-cell and eight-cell stages in each group). The colour scale bar was shown at the right of the heatmap. Blue indicated the lowest expression, and red indicated the highest expression; (D) the significantly changed epigenetic genes were detected by quantitative polymerase chain reaction in four-cell embryos. The experiment was independently repeated three times; (E) immunofluorescence of SETD2 in two-cell, four-cell, eight-cell and blastocyst. The nuclei (blue) were stained with DAPI. The experiment was repeated three times independently with five to 10 embryos per stage per group per replicate. scale bar = 50 μm. The negative control is presented in Supplementary Figure 4A; (F) fluorescence intensity analysis of SETD2; (G) immunofluorescence of KDM5B in fetal fibroblasts. The nuclei (blue) were stained with DAPI. The experiment was repeated three times independently. scale bar = 50 μm. The negative control is presented in Supplementary Figure 4D; (H) fluorescence intensity analysis of KDM5B. Data are presented as the mean ± standard error of the mean in panels D and F. The Solid line was the median and the dotted line was the interquartile range. T-tests were used to compare differences in panels D, H and F.