Phospho-Gli3 (Ser664) Antibody - #AF7449

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产品: 磷酸化 Gli3 (Ser664) 抗体
货号: AF7449
描述: Rabbit polyclonal antibody to Phospho-Gli3 (Ser664)
应用: WB IHC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: P10071
RRID: AB_2843889

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Gli3 (Ser664) Antibody detects endogenous levels of Gli3 only when phosphorylated at Ser664.
RRID:
AB_2843889
引用格式: Affinity Biosciences Cat# AF7449, RRID:AB_2843889.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACLS; DNA binding protein; GCPS; Gli 3; GLI family zinc finger 3; GLI Kruppel family member GLI 3; GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome); GLI Kruppel family member GLI3; GLI3; GLI3 C-terminally truncated form; GLI3 form of 190 kDa; GLI3 form of 83 kDa; GLI3 full length protein; GLI3-190; GLI3-83; GLI3_HUMAN; GLI3FL; Glioma associated oncogene family zinc finger 3; Oncogene GLI3; PAP A; PAPA 1; PAPA; PAPA1; PAPB; PHS; PPD IV; PPDIV; Transcriptional activator GLI3; Transcriptional repressor GLI3R; Zinc finger protein GLI 3; Zinc finger protein GLI3;

抗原和靶标

免疫原:

A synthesized peptide derived from human Gli3 around the phosphorylation site of Ser664.

基因/基因ID:
GLI3(2737)

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

文献引用

1). SMURF1 leads to the β-catenin signaling-mediated progression of esophageal squamous carcinoma by losing PATZ1-induced CCNG2 transcription. Biochemical pharmacology, 2024 (PubMed: 39617210) [IF=5.8]
2). Positive correlation between the nuclear expression of GPER and pGLI3 in prostate cancer tissues from patients with different Gleason scores. Frontiers in endocrinology, 2024 (PubMed: 38370352) [IF=3.9]

Application: IHC    Species: Human    Sample: tumor tissues

Figure 3 Expression of GLI3 and pGLI3 in tumoral and stromal prostate tissue in different grade groups. Representative images of GLI3 in the parenchyma (A, B) and stromal (C, D) PCa tumor tissues and pGLI3 in the tumoral (E, F) and stromal (G, H) PCa tumor tissues by immunohistochemistry with HE staining (x200). Scale bar 80 µm.

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