产品: 磷酸化 MKP1/2 (Ser296/Ser318) 抗体
货号: AF3243
描述: Rabbit polyclonal antibody to Phospho-MKP1/2 (Ser296/Ser318)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 45kDa; 39kD,43kD(Calculated).
蛋白号: P28562 | Q13115
RRID: AB_2834669

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-MKP1/2 (Ser296/Ser318) Antibody detects endogenous levels of MKP1/2 only when phosphorylated at Serine 296/318.
RRID:
AB_2834669
引用格式: Affinity Biosciences Cat# AF3243, RRID:AB_2834669.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CL 100; CL100; Dual Specificity Phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1_HUMAN; DUSP 1; Dusp1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein tyrosine phosphatase CL100; Protein-tyrosine phosphatase CL100; PTPN10; Serine/threonine specific protein phosphatase; VH1; Dual specificity phosphatase 4; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH2; DUS4_HUMAN; DUSP 4; Dusp4; HVH 2; HVH2; MAP kinase phosphatase 2; Mitogen activated protein kinase phosphatase 2; Mitogen-activated protein kinase phosphatase 2; MKP 2; MKP-2; MKP2; Serine/threonine specific protein phosphatase; TYP; Typ1; VH 2; VH1 homologous phosphatase 2; VH2;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P28562 DUS1_HUMAN:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

描述:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus.
序列:
MVMEVGTLDAGGLRALLGERAAQCLLLDCRSFFAFNAGHIAGSVNVRFSTIVRRRAKGAMGLEHIVPNAELRGRLLAGAYHAVVLLDERSAALDGAKRDGTLALAAGALCREARAAQVFFLKGGYEAFSASCPELCSKQSTPMGLSLPLSTSVPDSAESGCSSCSTPLYDQGGPVEILPFLYLGSAYHASRKDMLDALGITALINVSANCPNHFEGHYQYKSIPVEDNHKADISSWFNEAIDFIDSIKNAGGRVFVHCQAGISRSATICLAYLMRTNRVKLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC

MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHGTLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSATEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCAAEAASPSGPLRERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLHSPITTSPSC

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P28562/Q13115 作为底物

Site PTM Type Enzyme
K57 Acetylation PR:000007102 (EP300)
K57 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K122 Ubiquitination
S131 Phosphorylation
S140 Phosphorylation
T141 Phosphorylation
S146 Phosphorylation
K221 Ubiquitination
K230 Ubiquitination
K280 Ubiquitination
K289 Ubiquitination
S296 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S323 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S334 Phosphorylation P54646 (PRKAA2)
S359 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S364 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
Site PTM Type Enzyme
V2 Acetylation
K16 Ubiquitination
Y60 Phosphorylation
S64 Phosphorylation
T71 Phosphorylation
K78 Ubiquitination
Y102 Phosphorylation
Y108 Phosphorylation
S112 Phosphorylation
Y154 Phosphorylation
S159 Phosphorylation
K160 Ubiquitination
K311 Ubiquitination
S345 Phosphorylation
T356 Phosphorylation
T359 Phosphorylation
S386 Phosphorylation
S391 Phosphorylation

研究背景

功能:

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

翻译修饰:

Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.

蛋白家族:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

功能:

Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

翻译修饰:

Phosphorylation in the C-terminus by ERK1/2 inhibits proteasomal degradation and stabilizes the protein.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Hollow spherical complex composed of 24 subunits with pseudooctahedral symmetry, has a tetramer as the basic unit.

蛋白家族:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

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