Phospho-MAPK6/ERK3 (Ser189) Antibody - #AF7407

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产品: 磷酸化 MAPK6/ERK3 (Ser189) 抗体
货号: AF7407
描述: Rabbit polyclonal antibody to Phospho-MAPK6/ERK3 (Ser189)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q16659
RRID: AB_2843847

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-MAPK6/ERK3 (Ser189) Antibody detects endogenous levels of MAPK6/ERK3 only when phosphorylated at Ser189.
RRID:
AB_2843847
引用格式: Affinity Biosciences Cat# AF7407, RRID:AB_2843847.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ERK-3; ERK3; Extracellular signal regulated kinase 3; Extracellular signal regulated kinase p97; Extracellular signal-regulated kinase 3; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; Mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; MK06_HUMAN; p97 MAPK; p97-MAPK; PRKM6; Protein kinase mitogen activated 5; Protein kinase mitogen activated 6;

抗原和靶标

免疫原:

A synthesized peptide derived from human MAPK6/ERK3 around the phosphorylation site of Ser189.

基因/基因ID:
MAPK6(5597)

研究领域

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). HNF4G increases cisplatin resistance in lung adenocarcinoma via the MAPK6/Akt pathway. PeerJ, 2023 (PubMed: 36923501) [IF=2.3]

Application: WB    Species: human    Sample:

Figure 3: HNF4G promotes MAPK6 expression and MAPK6/Akt signaling pathway. (A) HNF4G binding peaks in the promoter of MAPK6 based on the ChIP-Seq data from the Encode database. (B) Correlation between HNF4G and MAPK6 expression in TCGA lung adenocarcinoma samples. (C) MAPK6 qRT-PCR results in HNF4G overexpression and knockdown cells. (D) Western blot analyses of HNF4G, MAPK6, p-MAPK6, Akt, and p-Akt(S473) in cells overexpressing or knocking down HNF4G. (E) Cisplatin sensitivity curves of control and HNF4G overexpression cells treated with or without Akt inhibitor.

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