Phospho-CDK2 (Thr160) Antibody - #AF3237

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产品: 磷酸化 CDK2 (Thr160) 抗体
货号: AF3237
描述: Rabbit polyclonal antibody to Phospho-CDK2 (Thr160)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P24941
RRID: AB_2834663

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-CDK2 (Thr160) Antibody detects endogenous levels of CDK2 only when phosphorylated at Threonine 160.
RRID:
AB_2834663
引用格式: Affinity Biosciences Cat# AF3237, RRID:AB_2834663.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cdc2 related protein kinase; cdc2-related protein kinase; CDC28; CDC2A; Cdk 2; CDK1; CDK2; CDK2_HUMAN; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2);

抗原和靶标

免疫原:

A synthesized peptide derived from human CDK2 around the phosphorylation site of Thr160.

基因/基因ID:
CDK2(1017)
描述:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

文献引用

1). Rictor regulates the vasculogenic mimicry of melanoma via the AKT-MMP-2/9 pathway. Journal of Cellular and Molecular Medicine, 2017 (PubMed: 28699701) [IF=5.3]

Application: WB    Species: human    Sample:

Fig. 3 Knockdown of Rictor inhibits melanoma cells proliferation and blocked the cell cycle in G2/M phase. (A) Cell viability of A375 and MUM-2B cells after Rictor knockdown evaluated by MTT assay (*P < 0.05, **P < 0.01). (B) Cell cycle of A375 and MUM-2B cells after Rictor knockdown examined by FCM. (C) The expression of p-CDK2 and p-Histone H3 induced by knockdown of Rictor.
2). Experimental study on the inhibitory effect of Halofuginone on NSCLC. European journal of pharmacology, 2025 (PubMed: 39710292) [IF=4.2]

Application: WB    Species: human    Sample: NSCLC cells

Fig. 3. HF induces autophagy, apoptosis, and cell cycle arrest in NSCLC cells. (A) Submicroscopic structure of cells using electron microscopy after 24 h of treatment with HF 60 nM. (B) Observation of intracellular autophagy using CYTO-ID fluorescence staining 24 h after treatment with HF. (C) Expression of autophagy-related proteins 24 h after treatment with HF. (D) Quantitative graph of autophagy-related protein expression. (E) Detection of apoptosis using flow cytometry after 72 h of HF treatment. (F) Statistical graph of apoptosis rates of HCC827 and H1975. (G) Expression of apoptosis-related proteins after 72 h of HF treatment. (H) Quantitative graph of apoptosis-related protein expression. (I) Flow cytometric detection of the cell cycle distribution after exposure to different concentrations of HF. (J) Quantitative statistical graphs of G1, S, and G2/M phases percentages. (K) Protein blot analysis of CDK2, pCDK2 expression. (L) Quantitative graph of CDK2, pCDK2 protein expression. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
3). T‐17, a novel cyclin‐dependent kinases/histone deacetylases dual inhibitor, induces cancer cells death through cell cycle arrest and apoptosis. DRUG DEVELOPMENT RESEARCH, 2022 (PubMed: 35844039) [IF=3.5]

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