产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3237, RRID:AB_2834663.
展开/折叠
Cdc2 related protein kinase; cdc2-related protein kinase; CDC28; CDC2A; Cdk 2; CDK1; CDK2; CDK2_HUMAN; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2);
抗原和靶标
- P24941 CDK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P24941 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
K6 | Acetylation | Uniprot | |
K6 | Ubiquitination | Uniprot | |
K9 | Ubiquitination | Uniprot | |
T14 | Phosphorylation | P30291 (WEE1) | Uniprot |
Y15 | Phosphorylation | P07948 (LYN) , P30291 (WEE1) | Uniprot |
Y19 | Phosphorylation | Uniprot | |
K20 | Ubiquitination | Uniprot | |
K24 | Ubiquitination | Uniprot | |
K33 | Sumoylation | Uniprot | |
K33 | Ubiquitination | Uniprot | |
T39 | Phosphorylation | P31749 (AKT1) | Uniprot |
S46 | Phosphorylation | P50613 (CDK7) | Uniprot |
T47 | Phosphorylation | Uniprot | |
K56 | Ubiquitination | Uniprot | |
K65 | Ubiquitination | Uniprot | |
K129 | Ubiquitination | Uniprot | |
T137 | Phosphorylation | Uniprot | |
K142 | Ubiquitination | Uniprot | |
T158 | Phosphorylation | Uniprot | |
Y159 | Phosphorylation | Uniprot | |
T160 | Phosphorylation | P24941 (CDK2) , P28482 (MAPK1) , Q8IZL9 (CDK20) , P50613 (CDK7) , P27361 (MAPK3) | Uniprot |
T165 | Phosphorylation | P50613 (CDK7) | Uniprot |
Y168 | Phosphorylation | P50613 (CDK7) | Uniprot |
K237 | Ubiquitination | Uniprot | |
K250 | Ubiquitination | Uniprot | |
K273 | Ubiquitination | Uniprot | |
K278 | Ubiquitination | Uniprot | |
K291 | Ubiquitination | Uniprot | |
R297 | Methylation | Uniprot |
翻译修饰 - P24941 作为激酶
Substrate | Site | Source |
---|---|---|
A1KXE4 (FAM168B) | T57 | Uniprot |
O00267 (SUPT5H) | S666 | Uniprot |
O00267 (SUPT5H) | T775 | Uniprot |
O00267 (SUPT5H) | T791 | Uniprot |
O00311 (CDC7) | T376 | Uniprot |
O00429 (DNM1L) | S616 | Uniprot |
O00571 (DDX3X) | T204 | Uniprot |
O14497 (ARID1A) | S363 | Uniprot |
O14497 (ARID1A) | S1320 | Uniprot |
O14497 (ARID1A) | S1322 | Uniprot |
O14686 (KMT2D) | T5374 | Uniprot |
O14757 (CHEK1) | S286 | Uniprot |
O14757 (CHEK1) | S301 | Uniprot |
O15014 (ZNF609) | T722 | Uniprot |
O15350-1 (TP73) | T86 | Uniprot |
O43395 (PRPF3) | T167 | Uniprot |
O43463 (SUV39H1) | S391 | Uniprot |
O43663-1 (PRC1) | T470 | Uniprot |
O43663-1 (PRC1) | T481 | Uniprot |
O60271 (SPAG9) | T586 | Uniprot |
O60504 (SORBS3) | T562 | Uniprot |
O60504 (SORBS3) | S563 | Uniprot |
O60934 (NBN) | S432 | Uniprot |
O75152 (ZC3H11A) | T321 | Uniprot |
O75179 (ANKRD17) | S2042 | Uniprot |
O75179 (ANKRD17) | S2044 | Uniprot |
O75179 (ANKRD17) | S2045 | Uniprot |
O75179 (ANKRD17) | S2401 | Uniprot |
O75469 (NR1I2) | S114 | Uniprot |
O75469 (NR1I2) | T133 | Uniprot |
O75469 (NR1I2) | T135 | Uniprot |
O75469 (NR1I2) | S167 | Uniprot |
O75469 (NR1I2) | S200 | Uniprot |
O75469 (NR1I2) | S350 | Uniprot |
O75533 (SF3B1) | T142 | Uniprot |
O75533 (SF3B1) | T211 | Uniprot |
O75533 (SF3B1) | T244 | Uniprot |
O75533 (SF3B1) | T248 | Uniprot |
O75533 (SF3B1) | S259 | Uniprot |
O75533 (SF3B1) | T261 | Uniprot |
O75533 (SF3B1) | T267 | Uniprot |
O75533 (SF3B1) | T313 | Uniprot |
O75533 (SF3B1) | T354 | Uniprot |
O75533 (SF3B1) | T426 | Uniprot |
O75533 (SF3B1) | T432 | Uniprot |
O75533 (SF3B1) | T434 | Uniprot |
O75533 (SF3B1) | T436 | Uniprot |
O75553 (DAB1) | S524 | Uniprot |
O75616 (ERAL1) | S173 | Uniprot |
O94776 (MTA2) | S435 | Uniprot |
O94776 (MTA2) | T438 | Uniprot |
O94776 (MTA2) | T439 | Uniprot |
O94776 (MTA2) | S440 | Uniprot |
O95251 (KAT7) | T88 | Uniprot |
O95295 (SNAPIN) | S133 | Uniprot |
P03372 (ESR1) | S104 | Uniprot |
P03372 (ESR1) | S106 | Uniprot |
P03372 (ESR1) | S118 | Uniprot |
P03372 (ESR1) | S294 | Uniprot |
P04183 (TK1) | S13 | Uniprot |
P04637 (TP53) | S313 | Uniprot |
P04637 (TP53) | S314 | Uniprot |
P04637 (TP53) | S315 | Uniprot |
P04637-1 (TP53) | S392 | Uniprot |
P06400 (RB1) | S230 | Uniprot |
P06400 (RB1) | S249 | Uniprot |
P06400 (RB1) | T252 | Uniprot |
P06400 (RB1) | T356 | Uniprot |
P06400 (RB1) | T373 | Uniprot |
P06400 (RB1) | S567 | Uniprot |
P06400 (RB1) | S608 | Uniprot |
P06400 (RB1) | S612 | Uniprot |
P06400 (RB1) | S807 | Uniprot |
P06400 (RB1) | S811 | Uniprot |
P06400 (RB1) | T821 | Uniprot |
P06400 (RB1) | T826 | Uniprot |
P06401 (PGR) | S20 | Uniprot |
P06401 (PGR) | S25 | Uniprot |
P06401 (PGR) | S162 | Uniprot |
P06401 (PGR) | S190 | Uniprot |
P06401 (PGR) | S213 | Uniprot |
P06401 (PGR) | S294 | Uniprot |
P06401 (PGR) | S400 | Uniprot |
P06401 (PGR) | T430 | Uniprot |
P06401 (PGR) | S554 | Uniprot |
P06401 (PGR) | S676 | Uniprot |
P06748 (NPM1) | S70 | Uniprot |
P06748 (NPM1) | T199 | Uniprot |
P06748 (NPM1) | T234 | Uniprot |
P08621 (SNRNP70) | S226 | Uniprot |
P08670 (VIM) | S55 | Uniprot |
P08670 (VIM) | S56 | Uniprot |
P10244-1 (MYBL2) | T266 | Uniprot |
P10244-1 (MYBL2) | S393 | Uniprot |
P10244-1 (MYBL2) | T405 | Uniprot |
P10244-1 (MYBL2) | T440 | Uniprot |
P10244-1 (MYBL2) | T444 | Uniprot |
P10244-1 (MYBL2) | S452 | Uniprot |
P10244-1 (MYBL2) | T487 | Uniprot |
P10244-1 (MYBL2) | T494 | Uniprot |
P10244-1 (MYBL2) | T515 | Uniprot |
P10244-1 (MYBL2) | T518 | Uniprot |
P10244 (MYBL2) | T520 | Uniprot |
P10244-1 (MYBL2) | S577 | Uniprot |
P10412 (HIST1H1E) | T18 | Uniprot |
P10644 (PRKAR1A) | S83 | Uniprot |
P10644-1 (PRKAR1A) | T209 | Uniprot |
P12268 (IMPDH2) | S122 | Uniprot |
P12270 (TPR) | S1676 | Uniprot |
P12270 (TPR) | T1677 | Uniprot |
P12270 (TPR) | S1679 | Uniprot |
P12272-1 (PTHLH) | T108 | Uniprot |
P12272 (PTHLH) | T121 | Uniprot |
P12956 (XRCC6) | T455 | Uniprot |
P13051 (UNG) | S12 | Uniprot |
P13639 (EEF2) | T435 | Uniprot |
P13639 (EEF2) | S595 | Uniprot |
P14859 (POU2F1) | S269 | Uniprot |
P14859 (POU2F1) | T270 | Uniprot |
P15172 (MYOD1) | S200 | Uniprot |
P15336 (ATF2) | T116 | Uniprot |
P15923 (TCF3) | S139 | Uniprot |
P15923 (TCF3) | S245 | Uniprot |
P15927 (RPA2) | S23 | Uniprot |
P15927 (RPA2) | S29 | Uniprot |
P15927 (RPA2) | S33 | Uniprot |
P16949 (STMN1) | S25 | Uniprot |
P16949 (STMN1) | S38 | Uniprot |
P17096 (HMGA1) | S36 | Uniprot |
P17480 (UBTF) | S389 | Uniprot |
P17480 (UBTF) | S484 | Uniprot |
P17676 (CEBPB) | T235 | Uniprot |
P17706 (PTPN2) | S304 | Uniprot |
P18858 (LIG1) | S51 | Uniprot |
P18858 (LIG1) | S76 | Uniprot |
P18858 (LIG1) | S91 | Uniprot |
P18858 (LIG1) | T195 | Uniprot |
P18858 (LIG1) | T197 | Uniprot |
P18858 (LIG1) | T233 | Uniprot |
P18887 (XRCC1) | S226 | Uniprot |
P18887 (XRCC1) | S229 | Uniprot |
P19338 (NCL) | S67 | Uniprot |
P19338 (NCL) | T76 | Uniprot |
P19338 (NCL) | T121 | Uniprot |
P20248 (CCNA2) | S154 | Uniprot |
P22234 (PAICS) | S27 | Uniprot |
P22674 (CCNO) | S81 | Uniprot |
P23258 (TUBG1) | S80 | Uniprot |
P23396 (RPS3) | T220 | Uniprot |
P23396 (RPS3) | T221 | Uniprot |
P23511-2 (NFYA) | S291 | Uniprot |
P23511-2 (NFYA) | S297 | Uniprot |
P23511-1 (NFYA) | S320 | Uniprot |
P23511-1 (NFYA) | S326 | Uniprot |
P23771 (GATA3) | T156 | Uniprot |
P24864 (CCNE1) | S387 | Uniprot |
P24864 (CCNE1) | T395 | Uniprot |
P24864 (CCNE1) | S399 | Uniprot |
P24928 (POLR2A) | S1882 | Uniprot |
P24928 (POLR2A) | T1884 | Uniprot |
P24928 (POLR2A) | T1885 | Uniprot |
P24928 (POLR2A) | T1915 | Uniprot |
P24928 (POLR2A) | S1917 | Uniprot |
P24941-1 (CDK2) | Y19 | Uniprot |
P24941 (CDK2) | T160 | Uniprot |
P25054 (APC) | S1360 | Uniprot |
P25098 (GRK2) | S670 | Uniprot |
P25205 (MCM3) | T722 | Uniprot |
P26358 (DNMT1) | S154 | Uniprot |
P28324 (ELK4) | T194 | Uniprot |
P28324 (ELK4) | S387 | Uniprot |
P28749 (RBL1) | S640 | Uniprot |
P28749 (RBL1) | S650 | Uniprot |
P29350 (PTPN6) | S591 | Uniprot |
P29374 (ARID4A) | S864 | Uniprot |
P29374 (ARID4A) | S1007 | Uniprot |
P29374 (ARID4A) | T1124 | Uniprot |
P29374 (ARID4A) | S1140 | Uniprot |
P29374 (ARID4A) | S1145 | Uniprot |
P29590 (PML) | S518 | Uniprot |
P29966 (MARCKS) | S26 | Uniprot |
P29966 (MARCKS) | S27 | Uniprot |
P29966 (MARCKS) | S118 | Uniprot |
P29966 (MARCKS) | S131 | Uniprot |
P29966 (MARCKS) | S132 | Uniprot |
P29966 (MARCKS) | T133 | Uniprot |
P29966 (MARCKS) | S134 | Uniprot |
P29966 (MARCKS) | S135 | Uniprot |
P29966 (MARCKS) | S147 | Uniprot |
P29966 (MARCKS) | T150 | Uniprot |
P30050 (RPL12) | S38 | Uniprot |
P30291 (WEE1) | S123 | Uniprot |
P30304 (CDC25A) | S263 | Uniprot |
P30307 (CDC25C) | S214 | Uniprot |
P31350 (RRM2) | S20 | Uniprot |
P31350 (RRM2) | T33 | Uniprot |
P31943 (HNRNPH1) | S104 | Uniprot |
P32322 (PYCR1) | S301 | Uniprot |
P32322 (PYCR1) | S303 | Uniprot |
P33981-1 (TTK) | T468 | Uniprot |
P33991 (MCM4) | S3 | Uniprot |
P33991 (MCM4) | T7 | Uniprot |
P33991 (MCM4) | T19 | Uniprot |
P33991 (MCM4) | S32 | Uniprot |
P33991 (MCM4) | T53 | Uniprot |
P33991 (MCM4) | S54 | Uniprot |
P33991 (MCM4) | T110 | Uniprot |
P33993 (MCM7) | S121 | Uniprot |
P33993 (MCM7) | S365 | Uniprot |
P35269 (GTF2F1) | T445 | Uniprot |
P35269 (GTF2F1) | T446 | Uniprot |
P35611 (ADD1) | S613 | Uniprot |
P35611 (ADD1) | T614 | Uniprot |
P38398-3 (BRCA1) | S393 | Uniprot |
P38398-8 (BRCA1) | S1450 | Uniprot |
P38398 (BRCA1) | S1497 | Uniprot |
P38432 (COIL) | S184 | Uniprot |
P38919 (EIF4A3) | T163 | Uniprot |
P38936 (CDKN1A) | S130 | Uniprot |
P42677 (RPS27) | S27 | Uniprot |
P46013 (MKI67) | S357 | Uniprot |
P46527 (CDKN1B) | T187 | Uniprot |
P48200 (IREB2) | S157 | Uniprot |
P48634-2 (PRRC2A) | T1340 | Uniprot |
P49454 (CENPF) | T154 | Uniprot |
P49454 (CENPF) | S156 | Uniprot |
P49459-2 (UBE2A) | S90 | Uniprot |
P49459 (UBE2A) | S120 | Uniprot |
P49736 (MCM2) | S13 | Uniprot |
P49736 (MCM2) | S27 | Uniprot |
P49736 (MCM2) | S40 | Uniprot |
P49736 (MCM2) | S41 | Uniprot |
P49736 (MCM2) | S53 | Uniprot |
P49736 (MCM2) | S108 | Uniprot |
P49736 (MCM2) | S139 | Uniprot |
P49790 (NUP153) | S386 | Uniprot |
P49790 (NUP153) | T388 | Uniprot |
P49915 (GMPS) | S313 | Uniprot |
P49915 (GMPS) | T318 | Uniprot |
P49916 (LIG3) | T191 | Uniprot |
P49916 (LIG3) | S210 | Uniprot |
P49916 (LIG3) | S913 | Uniprot |
P50570 (DNM2) | S764 | Uniprot |
P50613 (CDK7) | S164 | Uniprot |
P50613 (CDK7) | T170 | Uniprot |
P50750 (CDK9) | S90 | Uniprot |
P51649 (ALDH5A1) | T181 | Uniprot |
P52948 (NUP98) | S612 | Uniprot |
P54132 (BLM) | S175 | Uniprot |
P54198-1 (HIRA) | T555 | Uniprot |
P54198 (HIRA) | T583 | Uniprot |
P54198 (HIRA) | S584 | Uniprot |
P54198 (HIRA) | T586 | Uniprot |
P54198 (HIRA) | T588 | Uniprot |
P54198-1 (HIRA) | S687 | Uniprot |
P55196 (AFDN) | T1352 | Uniprot |
P55273 (CDKN2D) | S76 | Uniprot |
P55273 (CDKN2D) | T141 | Uniprot |
P55795 (HNRNPH2) | S104 | Uniprot |
P57740 (NUP107) | S10 | Uniprot |
P57740 (NUP107) | S11 | Uniprot |
P61978 (HNRNPK) | S216 | Uniprot |
P62136 (PPP1CA) | T320 | Uniprot |
P62324 (BTG1) | S159 | Uniprot |
P62913 (RPL11) | T47 | Uniprot |
P78317 (RNF4) | T26 | Uniprot |
P78317 (RNF4) | T112 | Uniprot |
P78347 (GTF2I) | T556 | Uniprot |
P78347 (GTF2I) | T558 | Uniprot |
P82970 (HMGN5) | T31 | Uniprot |
P84022-1 (SMAD3) | T8 | Uniprot |
P84022-1 (SMAD3) | T179 | Uniprot |
P84022-1 (SMAD3) | S204 | Uniprot |
P84022-1 (SMAD3) | S208 | Uniprot |
P84022-1 (SMAD3) | S213 | Uniprot |
Q00059 (TFAM) | T122 | Uniprot |
Q00059 (TFAM) | S124 | Uniprot |
Q00536 (CDK16) | S138 | Uniprot |
Q01167 (FOXK2) | S373 | Uniprot |
Q01167 (FOXK2) | S428 | Uniprot |
Q01196 (RUNX1) | S249 | Uniprot |
Q01196 (RUNX1) | S266 | Uniprot |
Q01196 (RUNX1) | T273 | Uniprot |
Q01196-8 (RUNX1) | S276 | Uniprot |
Q01196-8 (RUNX1) | S293 | Uniprot |
Q01196-8 (RUNX1) | T300 | Uniprot |
Q01196-8 (RUNX1) | S303 | Uniprot |
Q02363 (ID2) | S5 | Uniprot |
Q02535 (ID3) | S5 | Uniprot |
Q02539 (HIST1H1A) | T152 | Uniprot |
Q03252 (LMNB2) | S37 | Uniprot |
Q03252 (LMNB2) | T39 | Uniprot |
Q04323 (UBXN1) | S199 | Uniprot |
Q04323 (UBXN1) | S200 | Uniprot |
Q04323 (UBXN1) | S202 | Uniprot |
Q04637 (EIF4G1) | T647 | Uniprot |
Q05516 (ZBTB16) | S197 | Uniprot |
Q05516 (ZBTB16) | T282 | Uniprot |
Q05519 (SRSF11) | S207 | Uniprot |
Q05519 (SRSF11) | S209 | Uniprot |
Q05682 (CALD1) | T730 | Uniprot |
Q06830 (PRDX1) | T90 | Uniprot |
Q07820 (MCL1) | S64 | Uniprot |
Q07820 (MCL1) | T92 | Uniprot |
Q07820 (MCL1) | S121 | Uniprot |
Q07820 (MCL1) | T163 | Uniprot |
Q08050-2 (FOXM1) | T596 | Uniprot |
Q08050-1 (FOXM1) | T611 | Uniprot |
Q08050-3 (FOXM1) | T649 | Uniprot |
Q08999 (RBL2) | S413 | Uniprot |
Q08999 (RBL2) | T417 | Uniprot |
Q08999 (RBL2) | S639 | Uniprot |
Q08999 (RBL2) | T642 | Uniprot |
Q08999 (RBL2) | S662 | Uniprot |
Q08999 (RBL2) | S688 | Uniprot |
Q08999 (RBL2) | T694 | Uniprot |
Q08999 (RBL2) | S952 | Uniprot |
Q08999 (RBL2) | S1044 | Uniprot |
Q08999 (RBL2) | S1068 | Uniprot |
Q08999 (RBL2) | S1080 | Uniprot |
Q08999 (RBL2) | T1097 | Uniprot |
Q08999 (RBL2) | S1112 | Uniprot |
Q0VD86 (INCA1) | S23 | Uniprot |
Q0VD86 (INCA1) | T182 | Uniprot |
Q0VD86 (INCA1) | S191 | Uniprot |
Q12778 (FOXO1) | S249 | Uniprot |
Q12800 (TFCP2) | T258 | Uniprot |
Q12800 (TFCP2) | S289 | Uniprot |
Q12800 (TFCP2) | S291 | Uniprot |
Q12800 (TFCP2) | S309 | Uniprot |
Q12888 (TP53BP1) | T1609 | Uniprot |
Q12888 (TP53BP1) | T1612 | Uniprot |
Q12906 (ILF3) | T363 | Uniprot |
Q12906 (ILF3) | T364 | Uniprot |
Q12906 (ILF3) | T368 | Uniprot |
Q12906 (ILF3) | S382 | Uniprot |
Q12906 (ILF3) | S384 | Uniprot |
Q12948 (FOXC1) | T68 | Uniprot |
Q12959 (DLG1) | S158 | Uniprot |
Q12959 (DLG1) | S443 | Uniprot |
Q13118 (KLF10) | S206 | Uniprot |
Q13242 (SRSF9) | S216 | Uniprot |
Q13309 (SKP2) | S64 | Uniprot |
Q13330 (MTA1) | S522 | Uniprot |
Q13352 (ITGB3BP) | S33 | Uniprot |
Q13352 (ITGB3BP) | S46 | Uniprot |
Q13415 (ORC1) | S258 | Uniprot |
Q13415 (ORC1) | S273 | Uniprot |
Q13415 (ORC1) | T375 | Uniprot |
Q13416 (ORC2) | T116 | Uniprot |
Q13416 (ORC2) | T226 | Uniprot |
Q13428 (TCOF1) | S582 | Uniprot |
Q13428 (TCOF1) | S583 | Uniprot |
Q13428 (TCOF1) | S982 | Uniprot |
Q13428 (TCOF1) | T983 | Uniprot |
Q13573 (SNW1) | S224 | Uniprot |
Q13573 (SNW1) | S232 | Uniprot |
Q13573 (SNW1) | S234 | Uniprot |
Q14103 (HNRNPD) | T193 | Uniprot |
Q14194 (CRMP1) | T509 | Uniprot |
Q14195 (DPYSL3) | T507 | Uniprot |
Q14195 (DPYSL3) | T508 | Uniprot |
Q14195 (DPYSL3) | T509 | Uniprot |
Q14202 (ZMYM3) | T826 | Uniprot |
Q14207 (NPAT) | S775 | Uniprot |
Q14207 (NPAT) | S779 | Uniprot |
Q14207 (NPAT) | S1100 | Uniprot |
Q14207 (NPAT) | T1270 | Uniprot |
Q14207 (NPAT) | T1350 | Uniprot |
Q14247 (CTTN) | S405 | Uniprot |
Q14676 (MDC1) | T341 | Uniprot |
Q14676 (MDC1) | S1068 | Uniprot |
Q14676 (MDC1) | T1630 | Uniprot |
Q14807 (KIF22) | S462 | Uniprot |
Q14807 (KIF22) | T463 | Uniprot |
Q14839 (CHD4) | T529 | Uniprot |
Q14839 (CHD4) | S531 | Uniprot |
Q14980 (NUMA1) | T1776 | Uniprot |
Q14CB8 (ARHGAP19) | T476 | Uniprot |
Q14CB8 (ARHGAP19) | T478 | Uniprot |
Q15003 (NCAPH) | T49 | Uniprot |
Q15047 (SETDB1) | S1066 | Uniprot |
Q15398 (DLGAP5) | T342 | Uniprot |
Q15398 (DLGAP5) | T344 | Uniprot |
Q15554 (TERF2) | S365 | Uniprot |
Q15648 (MED1) | S1207 | Uniprot |
Q15648 (MED1) | T1215 | Uniprot |
Q15717 (ELAVL1) | S202 | Uniprot |
Q15796 (SMAD2) | T8 | Uniprot |
Q15813 (TBCE) | S495 | Uniprot |
Q15910 (EZH2) | T345 | Uniprot |
Q15910 (EZH2) | T416 | Uniprot |
Q15942 (ZYX) | S303 | Uniprot |
Q15942 (ZYX) | T306 | Uniprot |
Q15942 (ZYX) | S308 | Uniprot |
Q16204 (CCDC6) | S244 | Uniprot |
Q2NL82 (TSR1) | T490 | Uniprot |
Q32P44 (EML3) | T881 | Uniprot |
Q32P44 (EML3) | S883 | Uniprot |
Q4VCS5 (AMOT) | S847 | Uniprot |
Q4VCS5 (AMOT) | T848 | Uniprot |
Q4VCS5 (AMOT) | S852 | Uniprot |
Q4VCS5 (AMOT) | S855 | Uniprot |
Q53ET0 (CRTC2) | S433 | Uniprot |
Q53GL7 (PARP10) | T101 | Uniprot |
Q53H80 (AKIRIN2) | S18 | Uniprot |
Q53H80 (AKIRIN2) | S21 | Uniprot |
Q5JTV8 (TOR1AIP1) | S143 | Uniprot |
Q5JTZ9 (AARS2) | T673 | Uniprot |
Q5T5Y3 (CAMSAP1) | S1080 | Uniprot |
Q5TZA2-2 (CROCC) | S763 | Uniprot |
Q5TZA2 (CROCC) | S1460 | Uniprot |
Q6P1N0 (CC2D1A) | S208 | Uniprot |
Q6PKG0 (LARP1) | T303 | Uniprot |
Q6PKG0 (LARP1) | T649 | Uniprot |
Q6PKG0 (LARP1) | S774 | Uniprot |
Q6UB35 (MTHFD1L) | S357 | Uniprot |
Q6Y7W6-3 (GIGYF2) | S30 | Uniprot |
Q6Y7W6-3 (GIGYF2) | S614 | Uniprot |
Q71UM5 (RPS27L) | S27 | Uniprot |
Q7KZ85 (SUPT6H) | T1523 | Uniprot |
Q7Z2Z1 (TICRR) | T969 | Uniprot |
Q7Z2Z1 (TICRR) | S1001 | Uniprot |
Q7Z417 (NUFIP2) | T219 | Uniprot |
Q7Z417 (NUFIP2) | T220 | Uniprot |
Q86T82 (USP37) | S628 | Uniprot |
Q86U86 (PBRM1) | S25 | Uniprot |
Q86U86 (PBRM1) | S27 | Uniprot |
Q86U86 (PBRM1) | T28 | Uniprot |
Q86U86 (PBRM1) | S32 | Uniprot |
Q86VM9 (ZC3H18) | S613 | Uniprot |
Q86VM9 (ZC3H18) | S618 | Uniprot |
Q86VM9 (ZC3H18) | S795 | Uniprot |
Q86VM9 (ZC3H18) | T796 | Uniprot |
Q86WB0 (ZC3HC1) | S394 | Uniprot |
Q86WB0 (ZC3HC1) | S395 | Uniprot |
Q86XL3 (ANKLE2) | S528 | Uniprot |
Q86YP4 (GATAD2A) | S598 | Uniprot |
Q86YP4 (GATAD2A) | S600 | Uniprot |
Q8IWS0 (PHF6) | S154 | Uniprot |
Q8IWS0 (PHF6) | S155 | Uniprot |
Q8IXJ6-2 (SIRT2) | S331 | Uniprot |
Q8IXJ6 (SIRT2) | S368 | Uniprot |
Q8IXM2 (BAP18) | S96 | Uniprot |
Q8IXM2 (BAP18) | S106 | Uniprot |
Q8IXM2 (BAP18) | S110 | Uniprot |
Q8IXQ3 (C9orf40) | S67 | Uniprot |
Q8IXQ3 (C9orf40) | S69 | Uniprot |
Q8IXQ3 (C9orf40) | S71 | Uniprot |
Q8IYB3 (SRRM1) | S769 | Uniprot |
Q8IYB3 (SRRM1) | S773 | Uniprot |
Q8IZL8 (PELP1) | S477 | Uniprot |
Q8IZL8 (PELP1) | S991 | Uniprot |
Q8N3F8 (MICALL1) | T469 | Uniprot |
Q8N3F8 (MICALL1) | S470 | Uniprot |
Q8N3F8 (MICALL1) | S471 | Uniprot |
Q8N3F8 (MICALL1) | S619 | Uniprot |
Q8N3F8 (MICALL1) | S620 | Uniprot |
Q8N3F8 (MICALL1) | S621 | Uniprot |
Q8NBZ0 (INO80E) | S154 | Uniprot |
Q8NFC6 (BOD1L1) | S2905 | Uniprot |
Q8NFC6 (BOD1L1) | S2907 | Uniprot |
Q8NFC6 (BOD1L1) | S2908 | Uniprot |
Q8NFC6 (BOD1L1) | S2909 | Uniprot |
Q8TAP6 (CEP76) | S83 | Uniprot |
Q8TAP8 (PPP1R35) | S45 | Uniprot |
Q8TAP8 (PPP1R35) | S47 | Uniprot |
Q8TAP8 (PPP1R35) | S52 | Uniprot |
Q8WUM0 (NUP133) | S27 | Uniprot |
Q8WUM0 (NUP133) | T28 | Uniprot |
Q8WUM0 (NUP133) | S50 | Uniprot |
Q8WWY3 (PRPF31) | S445 | Uniprot |
Q8WWY3 (PRPF31) | S446 | Uniprot |
Q8WWY3 (PRPF31) | S450 | Uniprot |
Q8WWY3 (PRPF31) | S451 | Uniprot |
Q8WWY3 (PRPF31) | T455 | Uniprot |
Q8WXE1 (ATRIP) | S224 | Uniprot |
Q8WXE1 (ATRIP) | S239 | Uniprot |
Q8WYH8 (ING5) | T152 | Uniprot |
Q92547 (TOPBP1) | T848 | Uniprot |
Q92620 (DHX38) | S232 | Uniprot |
Q92620 (DHX38) | S234 | Uniprot |
Q92785 (DPF2) | T176 | Uniprot |
Q92785 (DPF2) | T248 | Uniprot |
Q92785 (DPF2) | S251 | Uniprot |
Q969V6 (MRTFA) | T573 | Uniprot |
Q96C19 (EFHD2) | S74 | Uniprot |
Q96C19 (EFHD2) | S76 | Uniprot |
Q96C36 (PYCR2) | T301 | Uniprot |
Q96C36 (PYCR2) | S303 | Uniprot |
Q96C36 (PYCR2) | S304 | Uniprot |
Q96E09 (FAM122A) | S143 | Uniprot |
Q96EA4 (SPDL1) | S513 | Uniprot |
Q96EA4 (SPDL1) | S515 | Uniprot |
Q96II8 (LRCH3) | S419 | Uniprot |
Q96KS0 (EGLN2) | S130 | Uniprot |
Q96RK0 (CIC) | T583 | Uniprot |
Q96RK0 (CIC) | S585 | Uniprot |
Q96T88 (UHRF1) | S661 | Uniprot |
Q96T88-2 (UHRF1) | S674 | Uniprot |
Q99459 (CDC5L) | S410 | Uniprot |
Q99459 (CDC5L) | T411 | Uniprot |
Q99504 (EYA3) | S266 | Uniprot |
Q99504 (EYA3) | T268 | Uniprot |
Q99504 (EYA3) | T269 | Uniprot |
Q99504 (EYA3) | S271 | Uniprot |
Q99607 (ELF4) | T643 | Uniprot |
Q99607 (ELF4) | S648 | Uniprot |
Q99626 (CDX2) | S283 | Uniprot |
Q99626 (CDX2) | S287 | Uniprot |
Q99626 (CDX2) | S291 | Uniprot |
Q99638 (RAD9A) | S328 | Uniprot |
Q99708 (RBBP8) | T315 | Uniprot |
Q99708 (RBBP8) | T847 | Uniprot |
Q99728 (BARD1) | S148 | Uniprot |
Q99728 (BARD1) | S251 | Uniprot |
Q99728 (BARD1) | S288 | Uniprot |
Q99728 (BARD1) | T299 | Uniprot |
Q99741 (CDC6) | S54 | Uniprot |
Q99741 (CDC6) | S74 | Uniprot |
Q99741 (CDC6) | S106 | Uniprot |
Q9BUJ2 (HNRNPUL1) | S716 | Uniprot |
Q9BUJ2 (HNRNPUL1) | S718 | Uniprot |
Q9BVC5 (C2orf49) | S193 | Uniprot |
Q9BVC5 (C2orf49) | T197 | Uniprot |
Q9BVC5 (C2orf49) | T198 | Uniprot |
Q9BXR0 (QTRT1) | S139 | Uniprot |
Q9BY44 (EIF2A) | S517 | Uniprot |
Q9BY44 (EIF2A) | T518 | Uniprot |
Q9BZL4 (PPP1R12C) | S509 | Uniprot |
Q9C0C2 (TNKS1BP1) | S435 | Uniprot |
Q9C0C2 (TNKS1BP1) | S437 | Uniprot |
Q9C0C2 (TNKS1BP1) | S691 | Uniprot |
Q9C0C9 (UBE2O) | S836 | Uniprot |
Q9C0D5 (TANC1) | S1564 | Uniprot |
Q9H1E3 (NUCKS1) | S181 | Uniprot |
Q9H211 (CDT1) | T29 | Uniprot |
Q9H211 (CDT1) | S31 | Uniprot |
Q9H2G4 (TSPYL2) | S20 | Uniprot |
Q9H2G4 (TSPYL2) | T340 | Uniprot |
Q9H3D4 (TP63) | S477 | Uniprot |
Q9H3D4 (TP63) | T491 | Uniprot |
Q9H3D4 (TP63) | S560 | Uniprot |
Q9H3P2 (NELFA) | T157 | Uniprot |
Q9H425 (C1orf198) | S37 | Uniprot |
Q9H6K1 (ILRUN) | S215 | Uniprot |
Q9H7L9 (SUDS3) | S234 | Uniprot |
Q9H7L9 (SUDS3) | S236 | Uniprot |
Q9H7L9 (SUDS3) | S237 | Uniprot |
Q9H7L9 (SUDS3) | T244 | Uniprot |
Q9H8Y8 (GORASP2) | T225 | Uniprot |
Q9H910 (JPT2) | S97 | Uniprot |
Q9H9C1 (VIPAS39) | S130 | Uniprot |
Q9H9C1 (VIPAS39) | T132 | Uniprot |
Q9NP87 (POLM) | S372 | Uniprot |
Q9NPF5 (DMAP1) | T445 | Uniprot |
Q9NPF5 (DMAP1) | S448 | Uniprot |
Q9NS91 (RAD18) | S99 | Uniprot |
Q9NSV4 (DIAPH3) | T19 | Uniprot |
Q9NTI5 (PDS5B) | S1369 | Uniprot |
Q9NTI5 (PDS5B) | T1370 | Uniprot |
Q9NXR1 (NDE1) | T228 | Uniprot |
Q9NYB0 (TERF2IP) | S203 | Uniprot |
Q9NYV6 (RRN3) | S44 | Uniprot |
Q9NZJ0 (DTL) | S428 | Uniprot |
Q9NZJ0 (DTL) | T429 | Uniprot |
Q9P270 (SLAIN2) | S48 | Uniprot |
Q9P2B4 (CTTNBP2NL) | S563 | Uniprot |
Q9P2D1 (CHD7) | S2471 | Uniprot |
Q9P2D1 (CHD7) | T2472 | Uniprot |
Q9P2N5 (RBM27) | T796 | Uniprot |
Q9P2N5 (RBM27) | S798 | Uniprot |
Q9UEY8 (ADD3) | S423 | Uniprot |
Q9UFC0 (LRWD1) | S243 | Uniprot |
Q9UFC0 (LRWD1) | S245 | Uniprot |
Q9UGP5 (POLL) | S167 | Uniprot |
Q9UGP5 (POLL) | S177 | Uniprot |
Q9UGP5 (POLL) | S230 | Uniprot |
Q9UGP5 (POLL) | T553 | Uniprot |
Q9UIF9 (BAZ2A) | S509 | Uniprot |
Q9UIF9 (BAZ2A) | S1183 | Uniprot |
Q9UIF9 (BAZ2A) | S1184 | Uniprot |
Q9UJU6 (DBNL) | S274 | Uniprot |
Q9UJU6 (DBNL) | S275 | Uniprot |
Q9UKM9 (RALY) | S135 | Uniprot |
Q9UKY1 (ZHX1) | T568 | Uniprot |
Q9ULW0 (TPX2) | T72 | Uniprot |
Q9ULX3 (NOB1) | T121 | Uniprot |
Q9UM11 (FZR1) | S40 | Uniprot |
Q9UM11 (FZR1) | T121 | Uniprot |
Q9UM11 (FZR1) | S151 | Uniprot |
Q9UM11 (FZR1) | S163 | Uniprot |
Q9UPN3 (MACF1) | T7213 | Uniprot |
Q9UQR0 (SCML2) | T305 | Uniprot |
Q9UQR0 (SCML2) | S511 | Uniprot |
Q9UQR0 (SCML2) | S590 | Uniprot |
Q9Y266 (NUDC) | S136 | Uniprot |
Q9Y266 (NUDC) | S139 | Uniprot |
Q9Y2J2 (EPB41L3) | T466 | Uniprot |
Q9Y2J2 (EPB41L3) | T467 | Uniprot |
Q9Y2J2 (EPB41L3) | S960 | Uniprot |
Q9Y2J2 (EPB41L3) | S962 | Uniprot |
Q9Y2K7 (KDM2A) | T550 | Uniprot |
Q9Y2W1 (THRAP3) | T874 | Uniprot |
Q9Y314 (NOSIP) | S166 | Uniprot |
Q9Y314 (NOSIP) | T168 | Uniprot |
Q9Y3Z3 (SAMHD1) | T592 | Uniprot |
Q9Y676 (MRPS18B) | S49 | Uniprot |
研究背景
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.
Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.
Nitrosylated after treatment with nitric oxide (DETA-NO).
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus>Cajal body. Cytoplasm. Endosome.
Note: Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).
Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop. Found in a complex with both SPDYA and CDKN1B/KIP1. Binds to RB1 and CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17. Interacts with CEBPA (when phosphorylated). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with cyclins A, B1, B3, D, or E. Interacts with CDK2AP2.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
文献引用
Application: WB Species: human Sample:
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