Phospho-CKII alpha (Thr360) Antibody - #AF7334

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产品: 磷酸化 CKII alpha (Thr360) 抗体
货号: AF7334
描述: Rabbit polyclonal antibody to Phospho-CKII alpha (Thr360)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
蛋白号: P68400
RRID: AB_2843774

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-CKII alpha (Thr360) Antibody detects endogenous levels of CKII alpha only when phosphorylated at Thr360.
RRID:
AB_2843774
引用格式: Affinity Biosciences Cat# AF7334, RRID:AB_2843774.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1; Casein kinase II alpha 1 subunit; Casein kinase II alpha subunit; Casein kinase II subunit alpha; CK II alpha; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CKIIalpha; CSK21_HUMAN; CSNK2A1;

抗原和靶标

免疫原:

A synthesized peptide derived from human CKII alpha around the phosphorylation site of Thr360.

基因/基因ID:
CSNK2A1(1457)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

文献引用

1). Gentiopicroside Ameliorates Diabetic Renal Tubulointerstitial Fibrosis via Inhibiting the AT1R/CK2/NF-κB Pathway. Frontiers in Pharmacology, 2022 (PubMed: 35814242) [IF=5.6]

Application: WB    Species: Rat    Sample: NRK-52E cells

FIGURE 6 GPS inhibited HG-induced activation of the CK2/NF-κB pathway in NRK-52E cells. (A) Effects of GPS on p65 distribution in the nucleus and cytoplasm were detected by Western blot. **p < 0.01 vs. Ctrl. ## p < 0.01 and # p < 0.05 vs. HG. (B) Effects of GPS on p65 distribution in the nucleus and cytoplasm were detected by immunofluorescence. Scale bar: 20 μm. (C) Effect of GPS on the NF-κB p65 DNA binding activity was detected by EMSA. (D) Effect of GPS on the NF-κB p65 transcriptional activity was detected by luciferase reporter assay. **p < 0.01 vs. Ctrl. ## p < 0.01 and # p < 0.05 vs. HG. (E,F) Protein levels of IκBα, p-IκBα, and p-CK2α after GPS intervention were detected by Western blot. **p < 0.01 and *p < 0.05 vs. Ctrl. ## p < 0.01 and # p < 0.05 vs. HG. **p < 0.01 vs. Ctrl. ## p < 0.01 and # p < 0.05 vs. HG. Data were expressed as mean ± SD.

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