Phospho-Cofilin (Ser3) Antibody - #AF3232

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产品: 磷酸化Cofilin (Ser3) 抗体
货号: AF3232
描述: Rabbit polyclonal antibody to Phospho-Cofilin (Ser3)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白ID: P23528
RRID: AB_2834658

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Phospho-Cofilin (Ser3) Peptide

1mg
相关下载
抗原肽/阻断肽MS报告
抗原肽/阻断肽HPLC报告
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Cofilin (Ser3) Antibody detects endogenous levels of Cofilin only when phosphorylated at Serine 3.
RRID:
AB_2834658
引用格式: Affinity Biosciences Cat# AF3232, RRID:AB_2834658.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3.

基因/基因ID:
CFL1(1072)
描述:
cofilin 1 a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG. JCI insight, 2024 (PubMed: 39361424) [IF=8.0]

Application: WB    Species: human    Sample:

Figure 5 Knockdown SIPA1L1 promoted phagocytosis in HTM cells. HTM cells were transfected with si-SIPA1L1 (20 μM) for 8 hours with or without TGF-β2 (5 ng/mL) for 48 hours. (A) Expression of cytoskeletal proteins (F-actin indicated by phalloidin staining, green; G-actin, red) is shown with fluorescence micrographs. (B) Phagocytic activity was evaluated by incubating pHrodo Red bioparticles with the cells for 3 hours. (C and D) Red fluorescence intensity of pHrodo bioparticles was quantified by ImageJ and FACS. (E–H) Western blots were used to assess RhoA protein level and phosphorylated CFL1 protein level. (I) The proposed SIPA1L1 signaling pathway associated with phagocytosis is shown schematically. All results used more than 3 individual HTM cell strains and data are expressed as mean ± SEM (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001 compared with control; #P < 0.05, ##P < 0.01, ###P < 0.001 compared with TGF-β2–treated group by 1-way ANOVA followed by Tukey’s multiple-comparison test. Scale bars: 30 μm. Each symbol in graphs represents the data from each experiment.
2). Baicalin Attenuates Panton-Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells. International journal of molecular sciences, 2023 (PubMed: 37833969) [IF=5.6]

Application: WB    Species: bovine    Sample: BMECs

Figure 3. Effects of rPVL on the regulation of RhoA/ROCK/LIMK/Cofilin and PI3K/AKT/GSK-3β signaling pathways and phosphorylation of cofilin and tau hyperphosphorylation in the rPVL-treated BMECs. Representative immunoblot bands for RhoA, p-ROCK2(Tyr722), ROCK2, p-LIMK1/2(Thr508/Thr505), p-cofilin (Ser3), and cofilin (A); p-PI3K (Tyr458/Tyr199), PI3K, p-AKT(Ser473), AKT, GSK-3β(Ser9), GSK-3β, p-tau (Ser396), and tau (B); GAPDH was used as a control. rPVL was used at a 100 ng/mL concentration. Data are expressed as mean ± standard deviation of three independent experiments. * 0.01 < p < 0.05, ** p < 0.01 (one-way ANOVA with Dunnett’s multiple comparison tests), ns: not significant.
3). Neochlorogenic acid anchors MCU-based calcium overload for cancer therapy. Food & Function, 2021 (PubMed: 34672304) [IF=5.1]
4). Function of connexin 43 and RhoA/LIMK2/Cofilin signaling pathway in transient changes of contraction and dilation of human umbilical arterial smooth muscle cells. The International Journal of Biochemistry & Cell Biology, 2022 (PubMed: 36330887) [IF=3.4]
5). Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling. Molecular biotechnology, 2024 (PubMed: 37946061) [IF=2.4]

Application: WB    Species: human    Sample: cervical cancer cells

Fig. 5 SFN regulates the expression of LIMK2, p-LIMK2, Cofilin, and p-Cofilin in cervical cancer cells. A The expression of B SFN, C LIMK2, D p-LIMK2, E Cofilin, and F p-Cofilin in Ca Ski cells with SFN overexpressed or knocked down were detected by western blotting. G The expression of H SFN, I LIMK2, J p-LIMK2, K Cofilin, and L p-Cofilin in SiHa cells with SFN overexpressed or knocked down were detected by western blotting. (* p 

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