Phospho-WASP (Tyr291) Antibody - #AF7304

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产品: 磷酸化 WASP (Tyr291) 抗体
货号: AF7304
描述: Rabbit polyclonal antibody to Phospho-WASP (Tyr291)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Sheep, Rabbit, Dog, Xenopus
蛋白号: P42768
RRID: AB_2843744

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-WASP (Tyr291) Antibody detects endogenous levels of WASP only when phosphorylated at Tyr291.
RRID:
AB_2843744
引用格式: Affinity Biosciences Cat# AF7304, RRID:AB_2843744.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Eczema thrombocytopenia; IMD2; SCNX; THC; THC1; Thrombocytopenia 1 (X linked); U42471; Was; WASp; WASP_HUMAN; Wiskott Aldrich syndrome (eczema thrombocytopenia); Wiskott Aldrich syndrome; Wiskott Aldrich syndrome protein; Wiskott-Aldrich syndrome protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human WASP around the phosphorylation site of Tyr291.

基因/基因ID:
WAS(7454)

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). MAPK4 inhibits the early aberrant activation of B cells in rheumatoid arthritis by promoting the IRF4-SHIP1 signaling pathway. Cell death & disease, 2025 (PubMed: 39863600) [IF=8.1]

Application: IF/ICC    Species: human    Sample:

Fig. 7: MAPK4 inhibits the activation of the PI3K signaling pathway in B cells of arthritis mice by activating the IRF4-SHIP1 pathway. A Western blot analysis of pSHIP1 and pIRF4 expression levels in B cells from WT and MAPK4 KO mice stimulated in vitro with sAg for 0, 5, 10, and 30 min. B Western blot analysis of SHIP1 expression in B cells from WT and MAPK4 KO mice. C Co-immunoprecipitation assay to analyze the interaction between IRF4 and MAPK4. D Western blot analysis of pIRF4, pSHIP1, pPI3K (p85), and pAKT expression levels in WT B cells stimulated in vitro with sAg for 0, 5, 10, and 30 min, with or without Vacquinol-1 treatment. Representative blots from three independent experiments are presented for all of the above. E–H Flow cytometry (E-G) and statistical (H) analysis of FO(E), T1(E), T2(E), MZ(F), and GC (G) B cell subpopulations in WT mice treated in vivo with or without Vacquinol-1 every other day for 14 days (n = 5). I–K Flow cytometry (I) and statistical analysis (J-K) of CD4 and CD8 T cells from the spleen and thymus in WT mice treated in vivo with or without Vacquinol-1 every other day for 14 days (n = 5). L, M Flow cytometric analysis of IL-10 and IL-6 expression levels in B cells from WT, CIA, and CIA mice treated in vitro with Vacquinol-1 for 1 h (n = 4). *P 

Application: WB    Species: human    Sample:

Fig. 7: MAPK4 inhibits the activation of the PI3K signaling pathway in B cells of arthritis mice by activating the IRF4-SHIP1 pathway. A Western blot analysis of pSHIP1 and pIRF4 expression levels in B cells from WT and MAPK4 KO mice stimulated in vitro with sAg for 0, 5, 10, and 30 min. B Western blot analysis of SHIP1 expression in B cells from WT and MAPK4 KO mice. C Co-immunoprecipitation assay to analyze the interaction between IRF4 and MAPK4. D Western blot analysis of pIRF4, pSHIP1, pPI3K (p85), and pAKT expression levels in WT B cells stimulated in vitro with sAg for 0, 5, 10, and 30 min, with or without Vacquinol-1 treatment. Representative blots from three independent experiments are presented for all of the above. E–H Flow cytometry (E-G) and statistical (H) analysis of FO(E), T1(E), T2(E), MZ(F), and GC (G) B cell subpopulations in WT mice treated in vivo with or without Vacquinol-1 every other day for 14 days (n = 5). I–K Flow cytometry (I) and statistical analysis (J-K) of CD4 and CD8 T cells from the spleen and thymus in WT mice treated in vivo with or without Vacquinol-1 every other day for 14 days (n = 5). L, M Flow cytometric analysis of IL-10 and IL-6 expression levels in B cells from WT, CIA, and CIA mice treated in vitro with Vacquinol-1 for 1 h (n = 4). *P 

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