Phospho-Phospholamban (Ser16/Thr17) Antibody - #AF7278

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产品: 磷酸化 Phospholamban (Ser16/Thr17) 抗体
货号: AF7278
描述: Rabbit polyclonal antibody to Phospho-Phospholamban (Ser16/Thr17)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P26678
RRID: AB_2843718

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Phospholamban (Ser16/Thr17) Antibody detects endogenous levels of Phospholamban only when phosphorylated at Ser16/Thr17.
RRID:
AB_2843718
引用格式: Affinity Biosciences Cat# AF7278, RRID:AB_2843718.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cardiac phospholamban; CMD1P; CMH18; PLB; Pln; PPLA_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Phospholamban around the phosphorylation site of Ser16/Thr17.

基因/基因ID:
PLN(5350)

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Protective role of triiodothyronine in sepsis‑induced cardiomyopathy through phospholamban downregulation. International journal of molecular medicine, 2025 (PubMed: 39821325) [IF=5.7]
2). Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib. Frontiers in Pharmacology, 2019 (PubMed: 31507414) [IF=5.6]

Application: WB    Species: mouse    Sample: myocardial

FIGURE 4 | Upregulation of miR-146a decreased the abnormal expression of cardiac PLN and ANK2 caused by SNT. (A) Pln and Ank2 mRNA levels in myocardium of mice with different treatments. Values are normalized to the level of GAPDH. (B) Representative Western Blot bands for PLN and p-PLN protein expression and corresponding summary data. Band densitometry was normalized to Tubulin.
3). Interaction between A-kinase anchoring protein 5 and protein kinase A mediates CaMKII/HDAC signaling to inhibit cardiomyocyte hypertrophy after hypoxic reoxygenation. Cellular signalling, 2023 (PubMed: 36565899) [IF=4.4]

Application: WB    Species: Rat    Sample: H9C2 cardiomyocytes

Fig. 5. Low affinity AKAP5 - PKA interaction increased CaMKII activation and cellular hypertrophy. (A) Co-localization of AKAP5 with PKA in H9C2 cardiomyocytes; Scale bar indicates 20 μm. (B) Co-localization of AKAP5 with PLN in H9C2 cardiomyocytes; Scale bar indicates 20 μm. (C) Immunoprecipitation of AKAP5 with PKA and PLN in H9C2 cardiomyocytes in each group; Two-tailed Student's t-test was performed, means ± SD (n = 3); *P < 0.05 and **P < 0.01 vs. the CON group; *P < 0.05 and **P < 0.01 vs. the H/R group; CON, normal control group; H/R, hypoxia-reoxygenation group; siAKAP5, siRNA-AKAP5 transfection group; AKAP5, A-kinase anchoring protein 5; PKA, protein kinase A; PLN, phospholamban.
4). A-kinase Anchoring Protein 5 Anchors Protein Kinase A to Mediate PLN/SERCA to Reduce Cardiomyocyte Apoptosis Induced by Hypoxia and Reoxygenation. Biochemistry and Cell Biology, 2022 (PubMed: 35041539) [IF=2.4]

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