Phospho-Synapsin I (Ser427) Antibody - #AF7245

>>赠JD卡 已结束>>
(1)   (6)  
产品: 磷酸化 Synapsin I (Ser427) 抗体
货号: AF7245
描述: Rabbit polyclonal antibody to Phospho-Synapsin I (Ser427)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P17600
RRID: AB_2843685

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Synapsin I (Ser427) Antibody detects endogenous levels of Synapsin I only when phosphorylated at Ser427.
RRID:
AB_2843685
引用格式: Affinity Biosciences Cat# AF7245, RRID:AB_2843685.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI;

抗原和靶标

免疫原:

A synthesized peptide derived from human Synapsin I around the phosphorylation site of Ser427.

基因/基因ID:
SYN1(6853)

文献引用

1). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience, 2022 (PubMed: 35368262) [IF=4.3]

Application: WB    Species: Mice    Sample: N2a cells

FIGURE 6 Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (A) CHIP knock-down protein was designed by siRNA in N2a cells. Approximately 5 μl Stub1-siRNA was able to achieve effective knock-down CHIP expression, and consequently the expression level of synapsin I S9 was decreased with no change in synaptic protein SNAP25 and PSD95 expression. (B) Statistical bar chart to show that CHIP, synapsin I, SNAP25, and PSD95 expression were present (n = 6, **p < 0.01, ***p < 0.001). (C) The dose of 5 μl Stub1-siRNA showed a decrease in the expression levels of ubiquitin and synapsin I and the phosphorylation levels of synapsin I S9, S427, and S605. (D) Statistical bar chart to show that ubiquitin, synapsin I, synapsin I S9, S427, and S605 expression were present (n = 6, *p < 0.05).

Application: WB    Species: mouse    Sample: N2a cells

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (B) Statistical bar chart to show that CHIP, synapsin I, SNAP25, and PSD95 expression were present (n = 6, **p < 0.01, ***p < 0.001).

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.