Phospho-PKM2 (Ser37) Antibody - #AF7231

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产品: 磷酸化 PKM2 (Ser37) 抗体
货号: AF7231
描述: Rabbit polyclonal antibody to Phospho-PKM2 (Ser37)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
蛋白号: P14618
RRID: AB_2843671

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-PKM2 (Ser37) Antibody detects endogenous levels of PKM2 only when phosphorylated at Ser37.
RRID:
AB_2843671
引用格式: Affinity Biosciences Cat# AF7231, RRID:AB_2843671.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CTHBP; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM_HUMAN; MGC3932; OIP 3; OIP-3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK muscle type; PK, muscle type; PK2; PK3; PKM; PKM2; pykm; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; pyruvate kinase PKM; Pyruvate kinase, muscle 2; TCB; THBP1; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone-binding protein 1; Tumor M2 PK; Tumor M2-PK;

抗原和靶标

免疫原:

A synthesized peptide derived from human PKM2 around the phosphorylation site of Ser37.

基因/基因ID:
PKM(5315)

研究领域

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis. Cell Death & Disease, 2022 (PubMed: 36344490) [IF=8.1]

Application: WB    Species: Mouse    Sample:

Fig. 6: Inhibition of EGFR phosphorylation suppresses glycolysis-dependent M1 polarization in macrophages. A–F BMDMs were treated with LPS (1 μg/mL) for 24 h with or without PD168393 (10 μM) pretreatment for 30 min. A RT-qPCR analysis of mRNA expression of IL-1β (n = 3). B RT-qPCR analysis of mRNA expression of iNOS (n = 3). C Western blot was used to detect the expression of iNOS. D iNOS expression on the surface of BMDM was analyzed by flow cytometry. E Percentage of iNOS -positive BMDM is shown (n = 3). F Mean fluorescence intensity (MFI) is shown (n = 3). G–I Macrophages were collected from bronchoalveolar lavage fluid of C57BL/6 mice subjected to CLP and were divided into sham-operated, CLP and CLP plus Erlotinib (100 mg/kg, gavage) pretreatmend for 2 h, and alveolar macrophages were identified with CD45 + CD11b + F4/80high. G iNOS expression on the surface of alveolar macrophage was analyzed by flow cytometry. H Percentage of iNOS-positive alveolar macrophage is shown (n = 9). I Mean fluorescence intensity (MFI) is shown (n = 9). J Cluster analysis of differentially expressed metabolites in RAW264.7 measured after treated with LPS (1 μg/mL) for 30 min with or without PD168393 (PD 10 μM) pretreatment for 30 min, when compared with control. Blue and red indicates down-or upregulation, respectively (n = 3 samples of each condition). K Schematic illustrating the metabolites that are decreased (blue) in PD168393 (10 μM) RAW264.7 cells at 24 h after LPS stimulation. L PD168393 (10 μM) pretreatment RAW264.7 cells exhibited a ~2 -fold decrease in lactate levels compared with LPS group (n = 3). M Representative western blots of HIF1-a, p-PKM2, PKM2, LDHA expression in RAW264.7 cells. The graphs depict mean ± SD based on three independent experiments.
2). Shikonin improves pulmonary vascular remodeling in monocrotaline‑induced pulmonary arterial hypertension via regulation of PKM2. Molecular Medicine Reports, 2023 (PubMed: 36734266) [IF=3.4]
3). Ochratoxin A induces reprogramming of glucose metabolism by switching energy metabolism from oxidative phosphorylation to glycolysis in human gastric epithelium GES-1 cells in vitro. TOXICOLOGY LETTERS, 2020 (PubMed: 32835834) [IF=2.9]

Application: WB    Species: human    Sample: GES-1 cells

Fig. 5. |OTA promotes phosphorylation and inhibits activity of PKM2. (A) The mRNA expression of PKM2 was evaluated by qRT-PCR in OTA treated cells. (B) Protein expression levels of PKM2 and p-PKM2 (Ser 37) were evaluated after treated with OTA by Western blot.

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