Phospho-eIF2 alpha (Tyr150) Antibody - #AF7188

Fig. 5. T promoted the unfolded protein response (UPR) by upregulating ZIP9 level in primary Sertoli cells exposed to 2605 MHz RF-EMR and H2O2. Western blotting was performed to detect the level of UPR proteins. The levels of P-IRE1, P-PERK, P-eIF2a and P-ATF6 were higher in the RF+H50 group than those in the RF+H50 +T100 group while they were lower in the RF+H50 +T100 + LVZIP9 group than those in the RF+H50 +T100 group. No significant difference was observed in these UPR proteins between the RF+H50 +T100 + LVZIP9 group and the RF+H50 +T100 + LVNC group. Each bar in histogram represents the Mean ± SD. Symbol, * : P＜0.05 between two groups at the ends of the line.

Figure 1. High UA levels inhibits FGF21 and Sirt1 expression levels and promotes endoplasmic reticulum stress in HUVECs. (A) mRNA expression levels of FGF21 and Sirt1 were determined using reverse transcription-quantitative PCR. (B) Protein expression levels of FGF21 and Sirt1 were determined using western blotting. (C) Protein levels of ATF4, CHOP and p-eIF2A/eIF2A were determined using western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 mg/dl UA control group. FGF21, fibroblast growth factor 21; UA, uric acid; Sirt1, sirtuin 1; CHOP, eIF2A, eukaryotic initiation factor 2; ATF4, activating transcription factor 4; p-, phosphorylated.

Figure 1.| High UA levels inhibits FGF21 and Sirt1 expression levels and promotes endoplasmic reticulum stress in HUVECs.(C) Protein levels of ATF4, CHOP and p‑eIF2A/eIF2A were determined using western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 mg/dl UA control group. FGF21, fibroblast growth factor 21; UA, uric acid; Sirt1,sirtuin 1; CHOP, eIF2A, eukaryotic initiation factor 2; ATF4, activating transcription factor 4; p‑, phosphorylated.