Phospho-PKR (Thr446) Antibody - #AF3216

Fig. 6 CX3CL1 deficiency inhibited cisplatin-induced ER stress and HIF1A/HO-1 signal activation in kidney tissues. A Western blot analysis of proteins in kidney tissues that are associated with ER stress. B Western blotting was conducted to evaluate the HIF1A and HO-1 protein expression patterns in renal tissues. C Representative micrographs of CHOP and nephrin staining in renal tissues. Scale bar = 10 μm. D Representative micrographs of HO-1 and nephrin staining in renal tissues. Scale bar = 10 μm. E Western blot analysis was conducted to analyze the podocyte expression profiles of proteins associated with ER stress. F Podocytes’ HIF1A and HO-1 expression profiles were evaluated by western blotting. G Representative micrographs of podocytes stained with eIF2α and p-eIF2α. Scale bar = 10 μm. H Representative micrographs of podocytes stained with HIF1A and HO-1. Scale bar = 10 μm. (ER, endoplasmic reticulum; HIF1A/HO-1, hypoxia inducible factor 1-alpha/heme oxygenase-1; CHOP, CCAAT enhancer binding protein-homologous protein; eIF2α, eukaryotic translation initiation factor 2A; P value was calculated by one-way analysis of variance and Tukey’s test.

Fig. 6 CX3CL1 deficiency inhibited cisplatin-induced ER stress and HIF1A/HO-1 signal activation in kidney tissues. A Western blot analysis of proteins in kidney tissues that are associated with ER stress. B Western blotting was conducted to evaluate the HIF1A and HO-1 protein expression patterns in renal tissues. C Representative micrographs of CHOP and nephrin staining in renal tissues. Scale bar = 10 μm. D Representative micrographs of HO-1 and nephrin staining in renal tissues. Scale bar = 10 μm. E Western blot analysis was conducted to analyze the podocyte expression profiles of proteins associated with ER stress. F Podocytes’ HIF1A and HO-1 expression profiles were evaluated by western blotting. G Representative micrographs of podocytes stained with eIF2α and p-eIF2α. Scale bar = 10 μm. H Representative micrographs of podocytes stained with HIF1A and HO-1. Scale bar = 10 μm. (ER, endoplasmic reticulum; HIF1A/HO-1, hypoxia inducible factor 1-alpha/heme oxygenase-1; CHOP, CCAAT enhancer binding protein-homologous protein; eIF2α, eukaryotic translation initiation factor 2A; P value was calculated by one-way analysis of variance and Tukey’s test.