Phospho-LDHA (Tyr239) Antibody - #AF7173

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产品: 磷酸化 LDHA (Tyr239) 抗体
货号: AF7173
描述: Rabbit polyclonal antibody to Phospho-LDHA (Tyr239)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 37kDa; 37kD(Calculated).
蛋白号: P00338
RRID: AB_2843613

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(90%), Zebrafish(%), Bovine(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
Phospho-LDHA (Tyr239) Antibody detects endogenous levels of LDHA only when phosphorylated at Tyr239.
RRID:
AB_2843613
引用格式: Affinity Biosciences Cat# AF7173, RRID:AB_2843613.
偶联:
Unconjugated. 130
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cell proliferation-inducing gene 19 protein; L lactate dehydrogenase A chain; Lactate dehydrogenase A; LDH A; LDH M; LDH muscle subunit; LDH1; LDH5; LDHA; PIG 19; PIG19; Renal carcinoma antigen NY-REN-59;

抗原和靶标

免疫原:
Uniprot:

P00338(LDHA_HUMAN) >>Visit HPA database.

基因/基因ID:
LDHA(3939)
序列:
MATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLRTPKIVSGKDYNVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPNCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVHKQVVESAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPVSTMIKGLYGIKDDVFLSVPCILGQNGISDLVKVTLTSEEEARLKKSADTLWGIQKELQF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Zebrafish
90
Chicken
90
Rabbit
90
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

翻译修饰:

ISGylated.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homotetramer.

蛋白家族:

Belongs to the LDH/MDH superfamily. LDH family.

研究领域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

文献引用

1). Trophoblast-derived Lactic Acid Orchestrates Decidual Macrophage Differentiation via SRC/LDHA Signaling in Early Pregnancy. International Journal of Biological Sciences, 2022 (PubMed: 35002512) [IF=8.2]

Application: WB    Species: Mouse    Sample: macrophages

Figure 8 Exogenous LA (20 mM) regulates PBMC-derived macrophage polarization through the HIF1-α/SRC/LDHA pathway. (A) ROS fluorescence intensity (n=3). (B) NAD+/NADH ratio (n = 3). (C-L) Representative data and quantification of HIF-1α, HIF-2α, p-SRC (Y418), SRC, p-LDHA (Y10), p-LDHA (Y239), LDHA, INOS, VEGF and ARG1 protein expression in macrophages treated with or without 20mM LA in the presence or absence of PX478 under normoxia and hypoxia (n = 3-6). Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01.

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