Phospho-LATS1 (Thr1079) Antibody - #AF7169

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产品: 磷酸化 LATS1 (Thr1079) 抗体
货号: AF7169
描述: Rabbit polyclonal antibody to Phospho-LATS1 (Thr1079)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
蛋白号: O95835
RRID: AB_2843609

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-LATS1 (Thr1079) Antibody detects endogenous levels of LATS1 only when phosphorylated at Thr1079.
RRID:
AB_2843609
引用格式: Affinity Biosciences Cat# AF7169, RRID:AB_2843609.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

h-warts; Large tumor suppressor homolog 1; LATS large tumor suppressor homolog 1; LATS1; LATS1_HUMAN; Serine threonine protein kinase LATS1; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts;

抗原和靶标

免疫原:

A synthesized peptide derived from human LATS1 around the phosphorylation site of Thr1079.

基因/基因ID:
LATS1(9113)

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

文献引用

1). Activation of MST1 protects filtration barrier integrity of diabetic kidney disease in mice through restoring the tight junctions of glomerular endothelial cells. Acta pharmacologica Sinica, 2024 (PubMed: 39643641) [IF=8.2]
2). LSR targets YAP to modulate intestinal Paneth cell differentiation. Cell reports, 2023 (PubMed: 37703178) [IF=7.5]
3). Luteolin ameliorates experimental pulmonary arterial hypertension via suppressing Hippo-YAP/PI3K/AKT signaling pathway. Frontiers in Pharmacology, 2021 (PubMed: 33935785) [IF=5.6]

Application: WB    Species: rat    Sample: muscle cell

FIGURE 5 Luteolin inhibits the increase of mRNA and protein expression of key molecules in Hippo/YAP signaling pathway induced by PDGF-BB. PASMCs were cultured in 1% serum medium for 24 h, then treated with different concentrations of luteolin for 1 h before the stimulation with PDGF-BB (20 ng/ml). (A) mRNA level of LATS1 (n = 3). (B) mRNA level of YAP (n = 3). (C) and (D) the expression of protein level of LATS1 and phosphorylated LATS1 (n = 5). (E) and (F) the expression of protein level of YAP and phosphorylated YAP (n = 3). Bars represent means ± SD of n = 3–6, #DMSO group vs PDGF + DMSO group, #p < 0.05, ##p < 0.01, ###p < 0.0001, ###p < 0.0001, ####p < 0.00001, *PDGF + DMSO group vs PDGF + LUT group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no significance. LATS1, large tumor suppressor one; LUT, luteolin; PASMCs, pulmonary arterial smooth muscle cells; PDGF-BB, platelet-derived growth factor-BB; YAP, yes-association protein.
4). Sema3G activates YAP and promotes VSMCs proliferation and migration via Nrp2/PlexinA1. Cellular signalling, 2023 (PubMed: 36720439) [IF=4.4]
5). Integrin-Linked Kinase in the Development of Gastric Tumors Induced by Helicobacter pylori: Regulation and Prevention Potential. Helicobacter, 2024 (PubMed: 38951739) [IF=4.3]

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