Phospho-IRE1 (Ser724) Antibody - #AF7150

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产品: 磷酸化 IRE1 (Ser724) 抗体
货号: AF7150
描述: Rabbit polyclonal antibody to Phospho-IRE1 (Ser724)
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O75460
RRID: AB_2843590

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-IRE1 (Ser724) Antibody detects endogenous levels of IRE1 only when phosphorylated at phospho S724.
RRID:
AB_2843590
引用格式: Affinity Biosciences Cat# AF7150, RRID:AB_2843590.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; Ern1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1; Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; Ern1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1;

抗原和靶标

免疫原:

A synthesized peptide derived from human IRE1 around the phosphorylation site of phospho S724.

基因/基因ID:
ERN1(2081)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

文献引用

1). Targeting ceramide transfer protein sensitizes AML to FLT3 inhibitors via a GRP78-ATF6-CHOP axis. Nature communications, 2025 (PubMed: 39905002) [IF=16.6]

Application: WB    Species: human    Sample: Molm13 cell

a Schematic representation of the unfolded protein response pathways. Expression of GRP78, ATF6, CHOP, phospho-IRE1α (p-IRE1α), XBP1, phospho-PERK (p-PERK), phospho-eIF2α (p-eIF2α) and ATF4 was determined by western blotting. Molm13 (b) or (c) MV4-11 cells were treated with HPA-12 (80 µM), Creno (6 µM) or their combination for 48 h. Protein expression levels of GRP78 (d), ATF6 (e) and CHOP (f) in (b, c). n = 3 independent experiments. Effects of the ERS inhibitor 4-PBA on the viability of Molm13 (g) and MV4-11 (h) cells. n = 3 independent experiments. Representative flow plots of annexin V/PI staining of Molm13 (i) and MV4-11 (j) cells in (g, h). k, l Statistical analysis of the number of apoptotic cells in (i, j). n = 3 independent experiments. The data are presented as the means ± SDs, and differences were compared by 2-tailed Student’s t tests. Multiple groups were analyzed with one-way ANOVA. ns: not significant, *P 
2). SelK promotes glioblastoma cell proliferation by inhibiting β-TrCP1 mediated ubiquitin-dependent degradation of CDK4. Journal of experimental & clinical cancer research : CR, 2024 (PubMed: 39155374) [IF=11.3]

Application: WB    Species: Human    Sample: LN229 and U251 cells

Fig. 8. Knockdown of SelK activates ER stress by releasing calcium through IP3R channels, thereby down-regulating SKP2. A, BSKP2 mRNA levels determined by qPCR. C Detection of major markers of endoplasmic reticulum stress (Western blot). D SKP2 protein expression was detected in LN229 (Nonsense) and U251 (Nonsense) cells after treatment with 3 μM TM for 0, 6, 12 and 24 h. E The protein expression of SKP2 and GRP78 were detected after treatment of LN229 (shSelK#1), LN229 (shSelK#2), U251 (shSelK#1) and U251 (shSelK#2) cells with 1 mM 4-PBA for 12 h. F LN229 (Nonsense) and U251 (Nonsense) cells were treated with 3 μM TM for 0, 6, 12, and 24 h, and then SKP2 mRNA levels were assessed (qPCR). G, HSKP2 mRNA levels measured by qPCR after 12 h of 1 mM 4-PBA exposure of LN229 (shSelK#1), LN229 (shSelK#2), U251 (shSelK#1), and U251 (shSelK#2) cells. I-L Intracellular calcium levels in LN229 and U251 cells with knockdown SelK and in counterpart control cells evaluated using Calbryte 630 staining, after pre-treatment with 2-APB (50 μM) and dantrolene (25 μM) for 2 h, and flow cytometry
3). PCPE-1, a brown adipose tissue-derived cytokine, promotes obesity-induced liver fibrosis. The EMBO journal, 2024 (PubMed: 39160276) [IF=9.4]
4). Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression. JOURNAL OF PINEAL RESEARCH, 2023 (PubMed: 36732085) [IF=8.3]
5). p20BAP31 promotes cell apoptosis via interaction with GRP78 and activating the PERK pathway in colorectal cancer. International journal of biological macromolecules, 2024 (PubMed: 38844291) [IF=7.7]
6). Menaquinone-4 prevents medication-related osteonecrosis of the jaw through the SIRT1 signaling-mediated inhibition of cellular metabolic stresses-induced osteoblast apoptosis. Free Radical Biology and Medicine, 2023 (PubMed: 37364692) [IF=7.1]
7). Aqueous Extract of Pepino Leaves Ameliorates Palmitic Acid-Induced Hepatocellular Lipotoxicity via Inhibition of Endoplasmic Reticulum Stress and Apoptosis. Antioxidants, 2021 (PubMed: 34204987) [IF=7.0]

Application: WB    Species: Human    Sample: HepG2 Cells

Figure 5 AEPL attenuated ER stress while PA exposure. (a) ER stress-related protein levels were analyzed by Western blotting. (b–d) GRP78, phosphorylated PERK, and phosphorylated IRE1αwere normalized to PERK, IRE1α, and β-actin of each sample. (e) SREBP-1 level in each group was normalized to β-actin. All data were presented as mean ± SD of at least three independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. PA-treated cells.
8). X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicity. Genes & diseases, 2023 (PubMed: 37692514) [IF=6.9]
9). NTRK1-mediated protection against manganese-induced neurotoxicity and cell apoptosis via IGF2 in SH-SY5Y cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37984302) [IF=6.9]
10). ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35724508) [IF=6.9]
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