Phospho-ULK1 (Ser555)[Ser556] Antibody - #AF7148

Figure 6. Vasn deletion induced autophagy by regulating the mTOR-ULK1 signaling pathway. (A) Pathway enrichment results of target genes of differential expression microRNAs, where the mTOR pathway is of the Top 1 that has been significantly enriched (red highlightened). (B) Immunohistochemical analysis of mTOR signaling protein expression in the liver of WT, Vasn+/−, and Vasn−/− mice; bar = 100 μm. (C) Quantitative integrated optical density (IOD) analysis of immunohistochemical staining from ten mice per group, and three photos per sample. ** p < 0.01, *** p < 0.001. (D) Vasn deletion significantly decreased the expression of phosphorylated mTOR (S2448) and increased expression of the phosphorylated ULK1 (S555), confirmed by Western blot analysis.

Figure 6. Vasn deletion induced autophagy by regulating the mTOR-ULK1 signaling pathway. (A) Pathway enrichment results of target genes of differential expression microRNAs, where the mTOR pathway is of the Top 1 that has been significantly enriched (red highlightened). (B) Immunohistochemical analysis of mTOR signaling protein expression in the liver of WT, Vasn+/−, and Vasn−/− mice; bar = 100 μm. (C) Quantitative integrated optical density (IOD) analysis of immunohistochemical staining from ten mice per group, and three photos per sample. ** p < 0.01, *** p < 0.001. (D) Vasn deletion significantly decreased the expression of phosphorylated mTOR (S2448) and increased expression of the phosphorylated ULK1 (S555), confirmed by Western blot analysis.

Figure 7. XJZD inhibits the AMPK/ULK1(ser317) pathway. (A) The expression of ULK1 was detected by immunofluorescence assay, magnification ×400, scale bar: 20 µm (n = 6). (B) Using ImageJ software to analyse the fluorescence intensity of ULK1. (C) Representative Western blot bands of AMPK and p-AMPK. (D) Protein levels of p-AMPK/AMPK. (E) The mRNA levels of ULK1. (F) Representative Western blot bands of ULK1, p-ULK1(ser555) and p-ULK1(ser317). (G, H) Protein levels of p-ULK1(ser555)/ULK1 and p-ULK1(ser317)/ULK1 (n = 3). These data are means ± SD, ##p < 0.01 and ###p < 0.001 when compared with the control group. *p < 0.05, **p < 0.01 and ***p < 0.001 when compared with the model group. ns: no significant difference.