产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3206, RRID:AB_2834638.
展开/折叠
CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; OTTHUMP00000019861; OTTHUMP00000019862; VCL; VINC; VINC_HUMAN; Vinculin;
抗原和靶标
- P18206 VINC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P18206 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T8 | Phosphorylation | Uniprot | |
S52 | Phosphorylation | Uniprot | |
K59 | Ubiquitination | Uniprot | |
K71 | Acetylation | Uniprot | |
K71 | Ubiquitination | Uniprot | |
C85 | S-Nitrosylation | Uniprot | |
S97 | Phosphorylation | Uniprot | |
Y100 | Phosphorylation | P12931 (SRC) | Uniprot |
S101 | Phosphorylation | Uniprot | |
Y107 | Phosphorylation | Uniprot | |
S112 | Phosphorylation | Uniprot | |
K173 | Acetylation | Uniprot | |
S207 | Phosphorylation | Uniprot | |
K216 | Ubiquitination | Uniprot | |
K219 | Ubiquitination | Uniprot | |
K228 | Ubiquitination | Uniprot | |
S252 | Phosphorylation | Uniprot | |
S260 | Phosphorylation | Uniprot | |
S272 | Phosphorylation | Uniprot | |
S275 | Phosphorylation | Uniprot | |
K276 | Acetylation | Uniprot | |
S288 | Phosphorylation | Uniprot | |
S290 | Phosphorylation | Uniprot | |
K308 | Ubiquitination | Uniprot | |
K316 | Ubiquitination | Uniprot | |
T324 | Phosphorylation | Uniprot | |
S345 | Phosphorylation | Uniprot | |
S346 | Phosphorylation | Uniprot | |
S357 | Phosphorylation | Uniprot | |
K366 | Ubiquitination | Uniprot | |
T378 | Phosphorylation | Uniprot | |
S380 | Phosphorylation | Uniprot | |
S383 | Phosphorylation | Uniprot | |
K386 | Ubiquitination | Uniprot | |
K387 | Ubiquitination | Uniprot | |
R409 | Methylation | Uniprot | |
K426 | Ubiquitination | Uniprot | |
S434 | Phosphorylation | Uniprot | |
S439 | Phosphorylation | Uniprot | |
T442 | Phosphorylation | Uniprot | |
S443 | Phosphorylation | Uniprot | |
K444 | Acetylation | Uniprot | |
K444 | Ubiquitination | Uniprot | |
K464 | Ubiquitination | Uniprot | |
K476 | Ubiquitination | Uniprot | |
K496 | Acetylation | Uniprot | |
T508 | Phosphorylation | Uniprot | |
R512 | Methylation | Uniprot | |
Y537 | Phosphorylation | Uniprot | |
K544 | Ubiquitination | Uniprot | |
C545 | S-Nitrosylation | Uniprot | |
S566 | Phosphorylation | Uniprot | |
S574 | Phosphorylation | Uniprot | |
S579 | Phosphorylation | Uniprot | |
K581 | Ubiquitination | Uniprot | |
K584 | Ubiquitination | Uniprot | |
S596 | Phosphorylation | Uniprot | |
S600 | Phosphorylation | Uniprot | |
T604 | Phosphorylation | Uniprot | |
T614 | Phosphorylation | Uniprot | |
R622 | Methylation | Uniprot | |
S637 | Phosphorylation | Uniprot | |
K639 | Ubiquitination | Uniprot | |
T643 | Phosphorylation | Uniprot | |
K655 | Ubiquitination | Uniprot | |
T657 | Phosphorylation | Uniprot | |
S664 | Phosphorylation | Uniprot | |
K666 | Ubiquitination | Uniprot | |
T672 | Phosphorylation | Uniprot | |
S677 | Phosphorylation | Uniprot | |
Y692 | Phosphorylation | Uniprot | |
T697 | Phosphorylation | Uniprot | |
K699 | Acetylation | Uniprot | |
K699 | Ubiquitination | Uniprot | |
K708 | Ubiquitination | Uniprot | |
T719 | Phosphorylation | Uniprot | |
K720 | Ubiquitination | Uniprot | |
S721 | Phosphorylation | Uniprot | |
S726 | Phosphorylation | Uniprot | |
K731 | Ubiquitination | Uniprot | |
T754 | Phosphorylation | Uniprot | |
S755 | Phosphorylation | Uniprot | |
K768 | Acetylation | Uniprot | |
K768 | Ubiquitination | Uniprot | |
K778 | Ubiquitination | Uniprot | |
K784 | Ubiquitination | Uniprot | |
S787 | Phosphorylation | Uniprot | |
K792 | Ubiquitination | Uniprot | |
S795 | Phosphorylation | Uniprot | |
K802 | Ubiquitination | Uniprot | |
S809 | Phosphorylation | Uniprot | |
K815 | Ubiquitination | Uniprot | |
S816 | Phosphorylation | Uniprot | |
S820 | Phosphorylation | Uniprot | |
Y822 | Phosphorylation | Uniprot | |
K862 | Ubiquitination | Uniprot | |
S972 | Phosphorylation | Uniprot | |
S981 | Phosphorylation | Uniprot | |
S982 | Phosphorylation | Uniprot | |
K983 | Ubiquitination | Uniprot | |
K992 | Acetylation | Uniprot | |
R993 | Methylation | Uniprot | |
S1002 | Phosphorylation | Uniprot | |
R1003 | Methylation | Uniprot | |
K1020 | Ubiquitination | Uniprot | |
C1053 | S-Nitrosylation | Uniprot | |
K1064 | Ubiquitination | Uniprot | |
K1070 | Acetylation | Uniprot | |
K1070 | Ubiquitination | Uniprot | |
S1080 | Phosphorylation | Uniprot | |
S1101 | Phosphorylation | Uniprot | |
S1113 | Phosphorylation | Uniprot | |
T1123 | Phosphorylation | Uniprot | |
T1130 | Phosphorylation | Uniprot | |
Y1133 | Phosphorylation | P12931 (SRC) | Uniprot |
研究背景
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).
Acetylated; mainly by myristic acid but also by a small amount of palmitic acid.
Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Adherens junction. Cell junction>Focal adhesion. Cytoplasm>Cytoskeleton. Cell membrane>Sarcolemma>Peripheral membrane protein>Cytoplasmic side.
Note: Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
Metavinculin is muscle-specific.
Exhibits self-association properties. Interacts with APBB1IP and NRAP (By similarity). Interacts with TLN1. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin (By similarity). Interacts with SYNM. Interacts with SORBS1 (By similarity). Interacts with CTNNA1. Binds to ACTN4; this interaction triggers conformational changes.
Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. In isoform 2 (metavinculin) a 68 residue insertion in the tail domain promotes actin severing instead of bundling. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly.
Belongs to the vinculin/alpha-catenin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.