Phospho-IGFBP3 (Ser183) Antibody - #AF3203

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产品: 磷酸化 IGFBP3 (Ser183) 抗体
货号: AF3203
描述: Rabbit polyclonal antibody to Phospho-IGFBP3 (Ser183)
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 30 kDa; 32kD(Calculated).
蛋白号: P17936
RRID: AB_2834635

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-IGFBP3 (Ser183) Antibody detects endogenous levels of IGFBP3 only when phosphorylated at Serine 183.
RRID:
AB_2834635
引用格式: Affinity Biosciences Cat# AF3203, RRID:AB_2834635.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acid stable subunit of the 140 K IGF complex; Binding protein 29; Binding protein 53; BP 53; BP53; Growth hormone dependent binding protein; IBP 3; IBP-3; IBP3; IBP3_HUMAN; IGF binding protein 3; IGF-binding protein 3; IGFBP 3; IGFBP-3; IGFBP3; Insulin Like Growth Factor Binding Protein 3; Insulin-like growth factor binding protein 3 precursor; Insulin-like growth factor-binding protein 3;

抗原和靶标

免疫原:
Uniprot:

P17936(IBP3_HUMAN) >>Visit HPA database.

基因/基因ID:
IGFBP3(3486)
表达:
P17936 IBP3_HUMAN:

Expressed by most tissues. Present in plasma.

描述:
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II.
序列:
MQRARPTLWAAALTLLVLLRGPPVARAGASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17936 作为底物

Site PTM Type Enzyme
T7 Phosphorylation
N116 N-Glycosylation
N136 N-Glycosylation
S138 Phosphorylation P68400 (CSNK2A1)
S140 Phosphorylation P68400 (CSNK2A1)
S145 Phosphorylation
S148 O-Glycosylation
S148 Phosphorylation
S151 O-Glycosylation
S151 Phosphorylation
S153 O-Glycosylation
S155 O-Glycosylation
S156 O-Glycosylation
S156 Phosphorylation
T157 O-Glycosylation
S161 O-Glycosylation
S170 Phosphorylation
S183 Phosphorylation P78527 (PRKDC)
Y190 Phosphorylation
S192 O-Glycosylation
S194 Phosphorylation P68400 (CSNK2A1)
T195 O-Glycosylation
T197 Phosphorylation
N199 N-Glycosylation
S201 Phosphorylation
Y210 Phosphorylation
S231 Phosphorylation
Y246 Phosphorylation
T277 O-Glycosylation

研究背景

功能:

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

翻译修饰:

Phosphorylated by FAM20C in the extracellular medium.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed by most tissues. Present in plasma.

亚基结构:

Interacts with XLKD1 (By similarity). Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219.

蛋白家族:

The thyroglobulin type-1 domain mediates interaction with HN.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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