Phospho-LPIN1 (Thr14) Antibody - #AF7085

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产品: 磷酸化 LPIN1 (Thr14) 抗体
货号: AF7085
描述: Rabbit polyclonal antibody to Phospho-LPIN1 (Thr14)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 99kDa; 99kD(Calculated).
蛋白号: Q14693
RRID: AB_2843525

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-LPIN1 (Thr14) Antibody detects endogenous levels of LPIN1 only when phosphorylated at Thr14.
RRID:
AB_2843525
引用格式: Affinity Biosciences Cat# AF7085, RRID:AB_2843525.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC=3.1.3.4; KIAA0188; Lipin-1; Lpin1; LPIN1_HUMAN; PAP1; Phosphatidate phosphatase LPIN1;

抗原和靶标

免疫原:
Uniprot:

Q14693(LPIN1_HUMAN) >>Visit HPA database.

基因/基因ID:
LPIN1(23175)
表达:
Q14693 LPIN1_HUMAN:

Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.

序列:
MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Sheep
90
Zebrafish
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14693 作为底物

Site PTM Type Enzyme
T14 Phosphorylation
K54 Ubiquitination
S106 Phosphorylation P42345 (MTOR)
S134 Phosphorylation
T135 Phosphorylation
T145 Phosphorylation
S148 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation
T175 Phosphorylation
S176 Phosphorylation
S187 Phosphorylation
S188 Phosphorylation
T249 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
S293 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
S434 Phosphorylation
S438 Phosphorylation P42345 (MTOR)
S449 Phosphorylation
T479 Phosphorylation
K480 Ubiquitination
T563 Phosphorylation
S580 Phosphorylation
K609 Ubiquitination
K636 Ubiquitination
K698 Ubiquitination
K707 Ubiquitination
K711 Ubiquitination
S713 Phosphorylation
Y717 Phosphorylation
K770 Ubiquitination
K776 Ubiquitination
K854 Ubiquitination
S887 Phosphorylation
S889 Phosphorylation

研究背景

功能:

Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity).

翻译修饰:

Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway. Phosphorylation is decreased by epinephrine. Phosphorylation may not directly affect the catalytic activity but may regulate the localization. Dephosphorylated by the CTDNEP1-CNEP1R1 complex (By similarity).

Sumoylated.

细胞定位:

Nucleus membrane. Cytoplasm>Cytosol. Endoplasmic reticulum membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.

亚基结构:

Interacts (via LXXIL motif) with PPARA. Interacts with PPARGC1A. Interaction with PPARA and PPARGC1A leads to the formation of a complex that modulates gene transcription. Interacts with MEF2C.

蛋白家族:

Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL), a transcriptional binding motif, which mediates interaction with PPARA.

Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif essential for phosphatidate phosphatase activity.

Belongs to the lipin family.

研究领域

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Long-term dietary DHA intervention prevents telomere attrition and lipid disturbance in telomerase-deficient male mice. European Journal of Nutrition (PubMed: 36859557) [IF=5.0]

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