Phospho-CEBP Beta (Thr235/Thr188) Antibody - #AF3202

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产品: 磷酸化 CEBP Beta (Thr235/Thr188) 抗体
货号: AF3202
描述: Rabbit polyclonal antibody to Phospho-CEBP Beta (Thr235/Thr188)
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Dog
蛋白号: P17676
RRID: AB_2834634

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-CEBP Beta (Thr235) Antibody detects endogenous levels of CEBP Beta only when phosphorylated at Thr235 in human, or Thr188 in mouse and rat.
RRID:
AB_2834634
引用格式: Affinity Biosciences Cat# AF3202, RRID:AB_2834634.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AGP/EBP; C EBP beta; C/EBP beta; C/EBP related protein 2; CCAAT Enhancer Binding Protein beta; CCAAT/enhancer-binding protein beta; CEBPB; CEBPB_HUMAN; CRP2; IL 6DBP; IL6DBP; Interleukin 6 dependent binding protein; LAP; Liver activator protein; Liver enriched transcriptional activator; NF IL6; NFIL6; Nuclear factor NF IL6; Nuclear factor NF-IL6; SF B; SFB; Silencer factor B; TCF-5; TCF5; Transcription factor 5;

抗原和靶标

免疫原:

A synthesized peptide derived from human C/EBP- beta around the phosphorylation site of Thr235.

基因/基因ID:
CEBPB(1051)
描述:
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). TMF inhibits extracellular matrix degradation by regulating the C/EBPβ/ADAMTS5 signaling pathway in osteoarthritis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38554527) [IF=6.9]

Application: WB    Species: Human    Sample: C28/I2 cells

Fig. 2. TMF attenuated the effect of IL-1β on C28/I2 cells. (A) A CCK-8 assay was performed to detect the cell viability. (B–G) The protein expressions of Aggrecan, ADAMTS5, C/EBPβ, p-C/EBPβ, caspase3, and cleaved-caspase3 in IL-1β-treated C28/I2 cells was detected by western blotting assays. (H-I) The apoptosis rate was detected by a flow cytometer. *P ˂ 0.05, **P ˂ 0.01, and ***P ˂ 0.001. TMF (Low), 5 μg/mL TMF; TMF (High), 20 μg/mL TMF.

Application: IHC    Species: Human    Sample: C28/I2 cells

Fig. 3. TMF decreased ECM degradation by inhibiting C/EBPβ expression in IL-1β-treated C28/I2 cells. (A–B) IHC assays were used to detect the altered expression of p-C/EBPβ. (C–D)The protein expression of C/EBPβ in LV-sh-C/EBPβ-infected C28/I2 cells was detected by western blotting assays. (E–J) Western blotting assays were used to detect the protein expression of Aggrecan, ADAMTS5, C/EBPβ, p-C/EBPβ, caspase3, and cleaved-caspase3 in LV-sh-C/EBPβ-infected C28/I2 cells treated with IL-1β. (K–L) The apoptosis rate was determined by a flow cytometer.

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