Phospho-Occludin (Tyr287) Antibody - #AF7069

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产品: 磷酸化 Occludin (Tyr287) 抗体
货号: AF7069
描述: Rabbit polyclonal antibody to Phospho-Occludin (Tyr287)
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: Q16625
RRID: AB_2843509

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Occludin (Tyr287) Antibody detects endogenous levels of Occludin only when phosphorylated at Tyr287.
RRID:
AB_2843509
引用格式: Affinity Biosciences Cat# AF7069, RRID:AB_2843509.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;

抗原和靶标

免疫原:

A synthesized peptide derived from human Occludin around the phosphorylation site of Tyr287.

基因/基因ID:
OCLN(100506658)

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Atorvastatin-induced intracerebral hemorrhage is inhibited by berberine in zebrafish. Journal of applied toxicology : JAT, 2024 (PubMed: 38639436) [IF=2.7]
2). Protective effects of lycium barbarum polysaccharides on blood-retinal barrier via ROCK1 pathway in diabetic rats. American Journal of Translational Research, 2023 (PubMed: 31737184) [IF=1.7]

Application: WB    Species: rat    Sample:

Figure 7. | Western blot assay for P-Occludin, ROCK1 and P-MLC protein expression. The Western blot was performed at 4, 8 and 12 weeks after administration. The relative expression of P-Occludin, ROCK1 and P-MLC was displayed in (A-C) respectively. (D) Western blot assay. *P<0.05, **P<0.01 vs. CON group; #P<0.05, ##P<0.01 vs. 8 w DM group.

Application: IHC    Species: rat    Sample:

Figure 6.| Immunohistochemistry (IHC) assay for P-Occludin and ROCK1 protein expression. The IHC assay was performed at 4, 8 and 12 weeks after administration. CON: control group treated with normal saline; DM: model group treated with normal saline; LBP: model group treated with LBP; F: model group treated with fasudil; F + LBP: model group treated with fasudil and LBP.

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