产品: 磷酸化 JunD (Ser255) 抗体
货号: AF3200
描述: Rabbit polyclonal antibody to Phospho-JunD (Ser255)
应用: WB IHC IF/ICC IP
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Dog, Chicken
分子量: 38kDa; 35kD(Calculated).
蛋白号: P17535
RRID: AB_2834632

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 200ul RMB¥ 3200 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(89%), Bovine(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-JunD (Ser255) Antibody detects endogenous levels of JunD only when phosphorylated at Serine 255.
RRID:
AB_2834632
引用格式: Affinity Biosciences Cat# AF3200, RRID:AB_2834632.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activator protein 1; AP 1; AP1; Jun D; jun D proto oncogene; Jund; JunD FL isoform; JUND_HUMAN; Transcription factor jun D; Transcription factor jun-D;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
JunD Transcription factor binding AP-1 sites. Binds DNA as a dimer. Interacts with MEN1; this interaction represses transcriptional activation. Belongs to the bZIP family. Jun subfamily.
序列:
METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSYAGGAGGAGGAATVAFAAEPVPFPPPPPPGALGPPRLAALKDEPQTVPDVPSFGESPPLSPIDMDTQERIKAERKRLRNRIAASKCRKRKLERISRLEEKVKTLKSQNTELASTASLLREQVAQLKQKVLSHVNSGCQLLPQHQVPAY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Chicken
100
Zebrafish
89
Xenopus
73
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17535 作为底物

Site PTM Type Enzyme
S27 Phosphorylation
T38 Phosphorylation
S43 Phosphorylation
T71 Phosphorylation
S90 Phosphorylation P45983 (MAPK8)
K97 Ubiquitination
S100 Phosphorylation P45983 (MAPK8) , P27361 (MAPK3) , P28482 (MAPK1)
T117 Phosphorylation P45983 (MAPK8)
K127 Ubiquitination
S131 Phosphorylation
K142 Ubiquitination
K240 Sumoylation
T245 Phosphorylation
S251 Phosphorylation
S255 Phosphorylation
S259 Phosphorylation
T265 Phosphorylation
S294 Phosphorylation
S305 Phosphorylation
T308 Phosphorylation
S312 Phosphorylation
T313 Phosphorylation
S315 Phosphorylation
K325 Ubiquitination
K327 Ubiquitination

研究背景

功能:

Transcription factor binding AP-1 sites.

翻译修饰:

Phosphorylated by MAP kinases MAPK8 and MAPK10; phosphorylation is inhibited in the presence of MEN1.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds DNA as a dimer (By similarity). Interacts (via MBM motif) with MEN1; this interaction represses transcriptional activation. Interacts with MAPK10; this interaction is inhibited in the presence of MEN1.

蛋白家族:

Belongs to the bZIP family. Jun subfamily.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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