Phospho-KLF5 (Ser303) Antibody - #AF7042

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产品: 磷酸化 KLF5 (Ser303) 抗体
货号: AF7042
描述: Rabbit polyclonal antibody to Phospho-KLF5 (Ser303)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 51kDa; 51kD(Calculated).
蛋白号: Q13887
RRID: AB_2843482

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
Phospho-KLF5 (Ser303) Antibody detects endogenous levels of KLF5 only when phosphorylated at Ser303.
RRID:
AB_2843482
引用格式: Affinity Biosciences Cat# AF7042, RRID:AB_2843482.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE binding protein 2; BTE-binding protein 2; BTEB 2; BTEB2; CKLF; Colon krueppel like factor; Colon krueppel-like factor; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal enriched krueppel like factor; Intestinal-enriched krueppel-like factor; KLF 5; Klf5; KLF5_HUMAN; Krueppel like factor 5; Krueppel-like factor 5; Kruppel like factor 5 intestinal; Transcription factor BTEB 2; Transcription factor BTEB2;

抗原和靶标

免疫原:
Uniprot:

Q13887(KLF5_HUMAN) >>Visit HPA database.

基因/基因ID:
KLF5(688)
表达:
Q13887 KLF5_HUMAN:

Expressed only in testis and placenta.

序列:
MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVNSQNIQPVRYNRRSNPDLEKRRIHYCDYPGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYRKHTGAKPFQCGVCNRSFSRSDHLALHMKRHQN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

翻译修饰:

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed only in testis and placenta.

亚基结构:

Interacts with WWP1.

蛋白家族:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the krueppel C2H2-type zinc-finger protein family.

文献引用

1). Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop. Cell death & disease, 2022 (PubMed: 36042208) [IF=8.1]

Application: WB    Species: Human    Sample: MDA-MB-231 cells

Fig. 4 DCC-2036 and AXL regulate the KLF5 protein level through Akt/GSK3β pathway. A DCC-2036 decreased the phosphorylation of AKT (Ser473), GSK3β (Ser9), and total KLF5, and increased the phosphorylation of KLF5 (Ser303). TNBC cells were treated with DCC-2036 for 48 h, followed by the WB assay. B Gas6 promoted the increase of p-AKT, p-GSK3β, and total KLF5, as well as the decrease of p-KLF5 in MDA-MB-231 cells and HS-578T cells. Cells were treated with Gas6 (200 ng/ml) for 0, 30, 60, 90, and 120 min, and then examined by WB.

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