产品: 磷酸化 JunB (Ser259) 抗体
货号: AF3198
描述: Rabbit polyclonal antibody to Phospho-JunB (Ser259)
应用: WB IHC IF/ICC IP
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Xenopus
分子量: 40kDa; 36kD(Calculated).
蛋白号: P17275
RRID: AB_2834630

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 50ul RMB¥ 1300 现货
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 200ul RMB¥ 3200 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(86%)
克隆:
Polyclonal
特异性:
Phospho-JunB (Ser259) Antibody detects endogenous levels of JunB only when phosphorylated at Serine 259.
RRID:
AB_2834630
引用格式: Affinity Biosciences Cat# AF3198, RRID:AB_2834630.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activator protein 1; AP 1; AP1; Jun B; Jun B proto oncogene; Jun B protooncogene; Junb; JunB proto oncogene; JunB protoncogene 9; JunB protooncogene; JUNB_HUMAN; Transcription factor jun B; Transcription factor jun-B; Transcription factor junB;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
JunB Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
序列:
MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGAGGGVTEEQEGFADGFVKALDDLHKMNHVTPPNVSLGATGGPPAGPGGVYAGPEPPPVYTNLSSYSPASASSGGAGAAVGTGSSYPTTTISYLPHAPPFAGGHPAQLGLGRGASTFKEEPQTVPEARSRDATPPVSPINMEDQERIKVERKRLRNRLAATKCRKRKLERIARLEDKVKTLKAENAGLSSTAGLLREQVAQLKQKVMTHVSNGCQLLLGVKGHAF

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
86
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17275 作为底物

Site PTM Type Enzyme
K4 Sumoylation
K33 Sumoylation
K36 Acetylation
K36 Sumoylation
K36 Ubiquitination
Y47 Phosphorylation
S49 Phosphorylation
K51 Sumoylation
Y68 Phosphorylation
S79 Phosphorylation Q16539 (MAPK14)
T102 Phosphorylation Q16539 (MAPK14) , P45983 (MAPK8)
T104 Phosphorylation Q16539 (MAPK14) , P45983 (MAPK8)
Y109 Phosphorylation
Y111 Phosphorylation
S117 Phosphorylation
T129 Phosphorylation
T153 Phosphorylation
Y173 Phosphorylation P00519 (ABL1)
Y182 Phosphorylation P00519 (ABL1)
Y188 Phosphorylation P00519 (ABL1)
S189 Phosphorylation
S237 Phosphorylation
T238 Phosphorylation
K240 Acetylation
K240 Sumoylation
K240 Ubiquitination
S251 Phosphorylation P49841 (GSK3B)
T255 Phosphorylation P49841 (GSK3B)
S259 Phosphorylation
K304 Sumoylation
K304 Ubiquitination
S311 Phosphorylation
S312 Phosphorylation
K325 Ubiquitination
T330 Phosphorylation

研究背景

功能:

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

翻译修饰:

Ubiquitinated by ITCH, leading to its degradation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds DNA as a homodimer or as a heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains).

蛋白家族:

Belongs to the bZIP family. Jun subfamily.

研究领域

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

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