| 产品: | 磷酸化 PGK1 (Tyr324) 抗体 |
| 货号: | AF8608 |
| 描述: | Rabbit polyclonal antibody to Phospho-PGK1 (Tyr324) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 45kDa; 45kD(Calculated). |
| 蛋白号: | P00558 |
| RRID: | AB_2843458 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8608, RRID:AB_2843458.
展开/折叠
Cell migration-inducing gene 10 protein; Epididymis secretory sperm binding protein Li 68p; HEL S 68p; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2;
抗原和靶标
Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.
- P00558 PGK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00558 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K6 | Acetylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Methylation | Uniprot | |
| K30 | Sumoylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| R39 | Methylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| C50 | S-Nitrosylation | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K75 | Acetylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| Y76 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K86 | Acetylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K91 | Acetylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| C99 | S-Nitrosylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| C108 | S-Nitrosylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| R123 | Methylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K156 | Acetylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K191 | Acetylation | Uniprot | |
| K191 | Methylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y196 | Phosphorylation | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K267 | Acetylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K275 | Acetylation | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| T288 | Phosphorylation | Uniprot | |
| K291 | Acetylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| K322 | Acetylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K323 | Acetylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K353 | Acetylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| R365 | Methylation | Uniprot | |
| C367 | S-Nitrosylation | Uniprot | |
| T378 | Phosphorylation | Uniprot | |
| C379 | S-Nitrosylation | Uniprot | |
| C380 | S-Nitrosylation | Uniprot | |
| K382 | Acetylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K388 | Acetylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot |
研究背景
Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.
Cytoplasm.
Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.
Monomer.
Belongs to the phosphoglycerate kinase family.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
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