MeCP2 Antibody - #AF9265

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产品: MeCP2 抗体
货号: AF9265
描述: Rabbit polyclonal antibody to MeCP2
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Rabbit, Dog
蛋白号: P51608
RRID: AB_2843443

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MeCP2 Antibody detects endogenous levels of total MeCP2.
RRID:
AB_2843443
引用格式: Affinity Biosciences Cat# AF9265, RRID:AB_2843443.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AUTSX 3; AUTSX3; DKFZp686A24160; Mbd 5; Mbd5; MECP 2; MeCP 2 protein; MeCP-2 protein; Mecp2; MECP2_HUMAN; Methyl CpG binding protein 2 (Rett syndrome); Methyl CpG binding protein 2; Methyl-CpG-binding protein 2; MRX 16; MRX 79; MRX16; MRX79; MRXS 13; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP 10; WBP10;

抗原和靶标

免疫原:

A synthesized peptide derived from human MeCP2, corresponding to a region within N-terminal amino acids.

基因/基因ID:
MECP2(4204)
描述:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

文献引用

1). The role of BDNF transcription in the antidepressant-like effects of 18β-glycyrrhetinic acid in a chronic social defeat stress model. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38851983) [IF=6.7]
2). The role of BDNF transcription in the antidepressant-like effects of 18β-glycyrrhetinic acid in a chronic social defeat stress model. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38851983) [IF=6.7]
3). Homeobox A9 is a novel mediator of vascular smooth muscle cell phenotypic switching and proliferation by regulating methyl-CpG binding protein 2. Cellular signalling, 2023 (PubMed: 37127144) [IF=4.4]
4). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=3.5]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
5). CircMAPK1 promotes the proliferation and migration of vascular smooth muscle cells through miR-22-3p/methyl-CpG binding protein 2 axis. Nutrition, metabolism, and cardiovascular diseases : NMCD, 2021 (PubMed: 34059381) [IF=3.3]

Application: IF/ICC    Species: mouse    Sample:

Figure 4| CircMAPK1 can bind with and regulate miR-22e3p.The protein level of MECP2 was detected by Western blot (H) After indicated treatment,the expression and distribution of MECP2 was detected by immunofluorescence. Scale bar Z 50 mm. Each experiment was repeated for three times and the results were presented as mean SD. ))p < 0.01, )))p < 0.001. MECP2, methyl-CpG binding protein 2.

Application: WB    Species: mouse    Sample:

Figure 4| CircMAPK1 can bind with and regulate miR-22e3p.(E) The mRNA level of MECP2 was detected by quantitative real-time PCR (FeG)

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