Phospho-Stathmin 1 (Ser25) Antibody - #AF3194

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产品: 磷酸化 Stathmin 1 (Ser25) 抗体
货号: AF3194
描述: Rabbit polyclonal antibody to Phospho-Stathmin 1 (Ser25)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 19kDa; 17kD(Calculated).
蛋白号: P16949
RRID: AB_2834626

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-Stathmin 1 (Ser25) Antibody detects endogenous levels of Stathmin 1 only when phosphorylated at Serine 25.
RRID:
AB_2834626
引用格式: Affinity Biosciences Cat# AF3194, RRID:AB_2834626.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C1orf215; Lag; LAP 18; LAP18; Leukemia associated phosphoprotein p18; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP 18; Op18; p18; p19; Phosphoprotein 19; Phosphoprotein p19; pp17; pp19; PR22; Pr22 protein; Prosolin; Protein Pr22; SMN; Stathmin; Stathmin1; STMN 1; Stmn1; STMN1_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P16949(STMN1_HUMAN) >>Visit HPA database.

基因/基因ID:
STMN1(3925)
表达:
P16949 STMN1_HUMAN:

Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

描述:
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment.
序列:
MASSDIQVKELEKRASGQAFELILSPRSKESVPEFPLSPPKKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREHEKEVLQKAIEENNNFSKMAEEKLTHKMEANKENREAQMAAKLERLREKDKHIEEVRKNKESKDPADETEAD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P16949 作为底物

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
S4 Phosphorylation
K9 Acetylation
K9 Ubiquitination
R14 Methylation
S16 Phosphorylation Q13153 (PAK1) , Q16566 (CAMK4) , Q13554 (CAMK2B) , P17612 (PRKACA) , Q9UQM7 (CAMK2A)
S25 Phosphorylation P53778 (MAPK12) , P45983 (MAPK8) , P24941 (CDK2) , P27361 (MAPK3) , Q15759 (MAPK11) , P05771 (PRKCB) , P45984 (MAPK9) , O15264 (MAPK13) , P06493 (CDK1) , Q16539 (MAPK14) , P53779 (MAPK10) , P28482 (MAPK1)
R27 Methylation
S28 Phosphorylation
K29 Acetylation
K29 Methylation
K29 Ubiquitination
S31 Phosphorylation
S38 Phosphorylation Q00535 (CDK5) , O15264 (MAPK13) , P45983 (MAPK8) , Q8TAS1 (UHMK1) , Q13153 (PAK1) , P05771 (PRKCB) , P06493 (CDK1) , P28482 (MAPK1) , P53779 (MAPK10) , P24941 (CDK2) , P45984 (MAPK9) , P27361 (MAPK3)
K41 Ubiquitination
K43 Ubiquitination
S46 Phosphorylation
K52 Acetylation
K52 Ubiquitination
K53 Acetylation
K53 Ubiquitination
K62 Ubiquitination
S63 Phosphorylation P17612 (PRKACA)
K70 Acetylation
K70 Ubiquitination
K75 Ubiquitination
K80 Acetylation
K80 Ubiquitination
K85 Ubiquitination
S94 Phosphorylation
K95 Acetylation
K95 Ubiquitination
K100 Acetylation
K100 Ubiquitination
T102 Phosphorylation
K104 Acetylation
K104 Ubiquitination
K109 Acetylation
K119 Acetylation
K119 Ubiquitination
K128 Acetylation
K128 Ubiquitination
R134 Methylation
K137 Acetylation
K137 Ubiquitination
K140 Ubiquitination
T146 Phosphorylation

研究背景

功能:

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

翻译修饰:

Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.

细胞定位:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

亚基结构:

Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST.

蛋白家族:

Belongs to the stathmin family.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). Cancer/testis antigen CAGE mediates osimertinib resistance in non-small cell lung cancer cells and predicts poor prognosis in patients with pulmonary adenocarcinoma. Scientific reports, 2023 (PubMed: 37735252) [IF=4.6]

Application: WB    Species: Human    Sample: lung cancer cells

Figure 3 Osimertinib resistance is correlated with the enhanced autophagy, invasion, migration, and growth rates. Cell lysates from the indicated cancer cells were subjected to immunoblot (a) and immunoprecipitation (b). The uncropped blots are shown in Supplementary Materials. Immunofluorescence staining of CAGE (c) and LC3 (d) was performed. Representative images of three independent experiments were shown. Significance determined by one-way ANOVA. ***, p 

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