Uba5 Antibody - #AF9220

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产品: Uba5 抗体
货号: AF9220
描述: Rabbit polyclonal antibody to Uba5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 45kDa; 45kD(Calculated).
蛋白号: Q9GZZ9
RRID: AB_2843410

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
Uba5 Antibody detects endogenous levels of total Uba5.
RRID:
AB_2843410
引用格式: Affinity Biosciences Cat# AF9220, RRID:AB_2843410.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5730525G14Rik; AW240750; FLJ23251; FLJ23251UBA5; MGC105565; ThiFP1; UBA5; UBA5 ubiquitin activating enzyme E1 homolog; UBA5_HUMAN; UBE1DC; UBE1DC1; Ubiquitin activating enzyme 5; Ubiquitin activating enzyme E1 domain containing 1; Ubiquitin activating enzyme E1 domain containing protein 1; Ubiquitin like modifier activating enzyme 5; Ubiquitin-activating enzyme 5; Ubiquitin-activating enzyme E1 domain-containing protein 1; Ubiquitin-like modifier-activating enzyme 5; UFM1 activating enzyme; UFM1-activating enzyme;

抗原和靶标

免疫原:
Uniprot:

Q9GZZ9(UBA5_HUMAN) >>Visit HPA database.

基因/基因ID:
UBA5(79876)
表达:
Q9GZZ9 UBA5_HUMAN:

Widely expressed.

序列:
MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Bovine
89
Dog
89
Chicken
88
Xenopus
78
Zebrafish
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

E1-like enzyme which activates UFM1 and SUMO2.

细胞定位:

Cytoplasm. Nucleus. Golgi apparatus.
Note: Localizes mainly in the cytoplasm, while it localizes to the nucleus in presence of SUMO2 (PubMed:18442052).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Interacts (via C-terminus) with UFC1. Interacts with UFM1.

蛋白家族:

Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.

文献引用

1). Heat-induced antigen retrieval utilizing modified Tris-EDTA buffer for reprobing of Western blots on nitrocellulose paper. Analytical Biochemistry, 2023 (PubMed: 37150424) [IF=2.6]

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