| 产品: | TLE4 抗体 |
| 货号: | AF9214 |
| 描述: | Rabbit polyclonal antibody to TLE4 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 83kDa; 84kD(Calculated). |
| 蛋白号: | Q04727 |
| RRID: | AB_2843404 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9214, RRID:AB_2843404.
展开/折叠
B lymphocyte gene 1; E(sp1) homolog Drosophila; E(spI); enhancer of split groucho 4; ESG; ESG4; GRG4; KIAA1261; TIM; TIM1; timeless (Drosophila) homolog; TLE4; Transducin like enhancer protein 4; transducin-like enhancer of split 4;
抗原和靶标
- Q04727 TLE4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQAQHLSHGHGLPVPLTPHPSGLQPPAIPPIGSSAGLLALSSALGGQSHLPIKDEKKHHDNDHQRDRDSIKSSSVSPSASFRGAEKHRNSADYSSESKKQKTEEKEIAARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLDKTRLLKKDAPISPASIASSSSTPSSKSKELSLNEKSTTPVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFGIVPHAGMNGELTSPGAAYAGLHNISPQMSAAAAAAAAAAAYGRSPVVGFDPHHHMRVPAIPPNLTGIPGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMENSNVEVLHVTKPDKYQLHLHESCVLSLKFAHCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q04727 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| R13 | Methylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| Y225 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| K237 | Acetylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K281 | Acetylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| K308 | Acetylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K322 | Acetylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| T334 | Phosphorylation | Uniprot | |
| R431 | Methylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| R644 | Methylation | Uniprot | |
| Y695 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| K721 | Acetylation | Uniprot | |
| K721 | Ubiquitination | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K765 | Sumoylation | Uniprot |
研究背景
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).
Phosphorylated. PAX5 binding increases phosphorylation (By similarity).
Ubiquitinated by XIAP/BIRC4.
Nucleus.
In all tissues examined, mostly in brain, and muscle.
Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Interacts with SIX3 and SIX6 (By similarity). Interacts with PAX2.
WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
Belongs to the WD repeat Groucho/TLE family.
限制条款
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