StIp1 Antibody - #AF9204

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产品: StIp1 抗体
货号: AF9204
描述: Rabbit polyclonal antibody to StIp1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 93kDa; 93kD(Calculated).
蛋白号: Q6IA86
RRID: AB_2843394

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(89%), Bovine(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
StIp1 Antibody detects endogenous levels of total StIp1.
RRID:
AB_2843394
引用格式: Affinity Biosciences Cat# AF9204, RRID:AB_2843394.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AU023723; Elongation protein 2 homolog (S. cerevisiae); Elongator acetyltransferase complex subunit 2; Elongator complex protein 2; elongator protein 2; ELP2; ELP2_HUMAN; Epl2; FLJ10879; SHINC 2; SHINC-2; SHINC2; signal transducer and activator of transcription 3 interacting protein 1; signal transducer and activator of transcription interacting protein 1; STAT3 interacting protein 1; Stat3 interacting protein; STAT3-interacting protein 1; STATIP 1; STATIP1; STATIP1 protein; StIP; StIP1;

抗原和靶标

免疫原:
Uniprot:

Q6IA86(ELP2_HUMAN) >>Visit HPA database.

基因/基因ID:
ELP2(55250)
序列:
MVAPVLETSHVFCCPNRVRGVLNWSSGPRGLLAFGTSCSVVLYDPLKRVVVTNLNGHTARVNCIQWICKQDGSPSTELVSGGSDNQVIHWEIEDNQLLKAVHLQGHEGPVYAVHAVYQRRTSDPALCTLIVSAAADSAVRLWSKKGPEVMCLQTLNFGNGFALALCLSFLPNTDVPILACGNDDCRIHIFAQQNDQFQKVLSLCGHEDWIRGVEWAAFGRDLFLASCSQDCLIRIWKLYIKSTSLETQDDDNIRLKENTFTIENESVKIAFAVTLETVLAGHENWVNAVHWQPVFYKDGVLQQPVRLLSASMDKTMILWAPDEESGVWLEQVRVGEVGGNTLGFYDCQFNEDGSMIIAHAFHGALHLWKQNTVNPREWTPEIVISGHFDGVQDLVWDPEGEFIITVGTDQTTRLFAPWKRKDQSQVTWHEIARPQIHGYDLKCLAMINRFQFVSGADEKVLRVFSAPRNFVENFCAITGQSLNHVLCNQDSDLPEGATVPALGLSNKAVFQGDIASQPSDEEELLTSTGFEYQQVAFQPSILTEPPTEDHLLQNTLWPEVQKLYGHGYEIFCVTCNSSKTLLASACKAAKKEHAAIILWNTTSWKQVQNLVFHSLTVTQMAFSPNEKFLLAVSRDRTWSLWKKQDTISPEFEPVFSLFAFTNKITSVHSRIIWSCDWSPDSKYFFTGSRDKKVVVWGECDSTDDCIEHNIGPCSSVLDVGGAVTAVSVCPVLHPSQRYVVAVGLECGKICLYTWKKTDQVPEINDWTHCVETSQSQSHTLAIRKLCWKNCSGKTEQKEAEGAEWLHFASCGEDHTVKIHRVNKCAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
89
Chicken
88
Xenopus
75
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with STAT3 and JAKs (By similarity).

蛋白家族:

Belongs to the WD repeat ELP2 family.

文献引用

1). Stress-induced phosphoprotein 1 restrains spinal cord ischaemia-reperfusion injury by modulating NF-κB signalling. Journal of Cellular and Molecular Medicine, 2021 (PubMed: 34734476) [IF=5.3]

Application: WB    Species: Rat    Sample: spinal cord

FIGURE 1 STIP1 expression increases firstly and then decreases in rats after SCII treatment. A, The rats undergo ischaemia by clamping abdominal aorta for 1 h and reperfusion for 24 h; B, Western blotting detects STIP1 expression in rat spinal cord specimens in absence or presence of SCII treatment; C, Immunohistochemical staining detects STIP1 expression in rat spinal cord specimens. The brown represents STIP1, and the STIP1-postive cells are indicated by arrows. Scale bar =50 μm. SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1. **p < 0.01, ***p < 0.001 versus the Sham group

Application: IF/ICC    Species: Rat    Sample: spinal cord

FIGURE 3 STIP1 ameliorates ischaemia/reperfusion-induced inflammation in rat spinal cord. A, Immunohistochemical staining determines the expression and location of Iba-1 and STIP1. The arrows indicate co-location of STIP1 and Iba-1. Scale bar =50 μm; B, ELISA detects TNF-α and IL-6 secretion in rat spinal cord specimens following SCII treatment and STIP1 overexpression; C, Western blotting determines IκBβ expression in rat spinal cord specimens; D, Western blotting determines the cytoplasmic and nuclear expression of NF-κB p65. NF-κB, nuclear factor kappa B; SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1; TNF, tumour necrosis factor. ***p < 0.001 versus the Sham group; #p < 0.05, ###p < 0.001 versus the SCII + LV group

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