StIp1 Antibody - #AF9204

FIGURE 1 STIP1 expression increases firstly and then decreases in rats after SCII treatment. A, The rats undergo ischaemia by clamping abdominal aorta for 1 h and reperfusion for 24 h; B, Western blotting detects STIP1 expression in rat spinal cord specimens in absence or presence of SCII treatment; C, Immunohistochemical staining detects STIP1 expression in rat spinal cord specimens. The brown represents STIP1, and the STIP1-postive cells are indicated by arrows. Scale bar =50 μm. SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1. **p < 0.01, ***p < 0.001 versus the Sham group

FIGURE 3 STIP1 ameliorates ischaemia/reperfusion-induced inflammation in rat spinal cord. A, Immunohistochemical staining determines the expression and location of Iba-1 and STIP1. The arrows indicate co-location of STIP1 and Iba-1. Scale bar =50 μm; B, ELISA detects TNF-α and IL-6 secretion in rat spinal cord specimens following SCII treatment and STIP1 overexpression; C, Western blotting determines IκBβ expression in rat spinal cord specimens; D, Western blotting determines the cytoplasmic and nuclear expression of NF-κB p65. NF-κB, nuclear factor kappa B; SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1; TNF, tumour necrosis factor. ***p < 0.001 versus the Sham group; #p < 0.05, ###p < 0.001 versus the SCII + LV group