Phospho-CREB (Ser133) Antibody - #AF3189

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产品: 磷酸化 CREB (Ser133) 抗体
货号: AF3189
描述: Rabbit polyclonal antibody to Phospho-CREB (Ser133)
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P16220
RRID: AB_2834621

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Serine 133.
RRID:
AB_2834621
引用格式: Affinity Biosciences Cat# AF3189, RRID:AB_2834621.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Active transcription factor CREB; cAMP response element binding protein 1; cAMP response element binding protein; cAMP responsive element binding protein 1; cAMP-responsive element-binding protein 1; CREB; CREB-1; CREB1; CREB1_HUMAN; Cyclic AMP-responsive element-binding protein 1; MGC9284; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; Transactivator protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human CREB around the phosphorylation site of Ser133.

基因/基因ID:
CREB1(1385)
描述:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian rhythm.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

文献引用

1). Targeting P2Y14R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis. Nature communications, 2024 (PubMed: 38453952) [IF=16.6]
2). Neuropeptide Precursor VGF Promotes Neuroendocrine Differentiation and Cancer-Associated Fibroblast Activation in Small Cell Lung Cancer. Cancer research, 2025 (PubMed: 40857617) [IF=12.5]

Application: WB    Species: human    Sample: NCI-H82 cell

Figure 3. VGF drives NE differentiation of SCLC-A by activating CREB signaling and upregulating ASCL1. A, Volcano plot showing DEGs in NCI-H128 cells following VGF knockdown (shVGF) compared with negative control (NC). Down, downregulated; FC, fold change; NotSig, not significant; Up, upregulated. B, Venn diagram showing overlapping downregulated genes in NCI-H128 cells after VGF knockdown and in non–SCLC-A cells compared with SCLC-A cells, using data from the Cancer Cell Line Encyclopedia database. C, Western blot analysis of VGF, pCREB, CREB, and TOX3 in NCI-H128 cells following VGF knockdown. D, Co-IP assay demonstrating the interaction between CREB and TOX3 after TLQP-21 (10 µmol/L) stimulation for 24 hours. E, Comparison of potential transcription factors for ASCL1 from ChIP-seq databases and transcription factor prediction databases. F, ChIP–qPCR analysis of CREB binding to the ASCL1 promoter following TLQP-21 (10 µmol/L) stimulation for 24 hours. G, Predicted CREB-binding motif on the ASCL1 promoter using the JASPAR database, with the mutant site highlighted in red. H, Dual-luciferase reporter assay assessing CREB-driven transcription of ASCL1 after mutating the binding motif and TLQP-21 (10 µmol/L) stimulation for 24 hours. I, Dual-luciferase reporter assay assessing CREB-driven transcription of ASCL1 following treatment with SB290157 (4 µmol/L) or 666-15 (100 nmol/L) alongside TLQP-21 (10 µmol/L) stimulation for 24 hours. MUT, mutant; WT, wild-type. J, Western blot analysis of pCREB, CREB, ASCL1, CHGA, and SYP in NCI-H82 cells following TLQP-21 (10 µmol/L) stimulation or VGF overexpression. K, Western blot analysis of VGF, pCREB, CREB, ASCL1, CHGA, and SYP in NCI-H82 cells following CREB overexpression. L, RT-qPCR analysis of ASCL1, CHGA, and SYP mRNA levels in NCI-H128 and NCI-H82 cells following TLQP-21 (10 µmol/L) stimulation, SB290157 (4 µmol/L), or 666-15 (100 nmol/L) treatment for 24 hours. M, Western blot analysis of pCREB, CREB, ASCL1, CHGA, and SYP in SCLC cells following TLQP-21 (10 µmol/L) stimulation, SB290157 (4 µmol/L), or 666-15 (100 nmol/L) treatment for 24 hours. N, Western blot analysis of pCREB, CREB, ASCL1, CHGA, and SYP in SCLC cells treated with CM from NCI-H128 cells with high VGF expression, SB290157 (4 µmol/L), or 666-15 (100 nmol/L) for 24 hours. Differences between groups were assessed using the unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 3. Data are presented as the means ± SEM.
3). The brain-protective mechanism of fecal microbiota transplantation from young donor mice in the natural aging process via exosome, gut microbiota, and metabolomics analyses. Pharmacological research, 2024 (PubMed: 39053865) [IF=9.1]

Application: WB    Species: Mouse    Sample:

Fig. 3. The cerebral protection effects of FMT in mice against age-associated proteins expression levels. (A, B) Quantified protein levels of the cell cycle arrest related proteins of p53, p21, p16/p14, and Rb in mice hippocampus tissues. Data are shown as the mean ± SD, n = 3 per group. Significance was determined by one-way ANOVA followed by the Dunnett T3 test for comparison of multiple groups, *P < 0.05, **P < 0.01, and ***P < 0.001. (C, D) Quantified protein levels of the DNA damage-related protein ATM, and the cognitive-related proteins synapsin I, synaptophysin and PSD95 in mice hippocampus tissues. Data are shown as the mean ± SD, n = 3 per group. Significance was determined by one-way ANOVA followed by the Dunnett T3 test for comparison of multiple groups, *P < 0.05, **P < 0.01, and ***P < 0.001. (E, F) Quantified protein levels of the cell senescence-related proteins CREB, p-CREB, ERK, p-ERK, AKT, p-AKT in mice hippocampus tissues. Data are shown as the mean ± SD, n = 3 per group. Significance was determined by one-way ANOVA followed by the Dunnett T3 test for comparison of multiple groups, *P < 0.05, **P < 0.01, and ***P < 0.001. (G, H) Quantified protein levels of the c-H2AX and TP53BP1 proteins in bone marrow mesenchymal stem cells. Data are shown as the mean ± SD, n = 3 per group. Significance was determined by one-way ANOVA followed by the Dunnett T3 test for comparison of multiple groups, *P < 0.05, **P < 0.01, and ***P < 0.001. (I, J) Quantified protein levels of the β-galactosidase, c-H2AX, and TP53BP1 proteins in in mice hippocampus tissues. Data are shown as the mean ± SD, n = 3 per group. Significance was determined by one-way ANOVA followed by the Dunnett T3 test for comparison of multiple groups, *P < 0.05, **P < 0.01, and ***P < 0.001.
4). CGRP-releasing PLGA/nHA/GO composite microspheres enhance distraction osteogenesis via activation of the cAMP/PKA/CREB pathway. Materials today. Bio, 2025 (PubMed: 40893374) [IF=8.7]

Application: WB    Species: Rat    Sample:

Fig. 5. Molecular Mechanism Analysis (A) Intracellular cAMP concentration measured by ELISA after 30 min of treatment, normalized to total protein content (n = 3). (B–D) Western blot analysis and quantitative assessment of PKA and CREB phosphorylation levels. (B) Representative Western blot images showing p-PKA (Thr197), total PKA, p-CREB (Ser133), total CREB, and GAPDH expression. (C) Quantitative analysis of p-PKA/total PKA ratio. (D) Quantitative analysis of p-CREB/total CREB ratio. (E) qRT-PCR analysis of osteogenic-related gene mRNA expression after 7 days of osteogenic induction. Relative expression levels of Runx2, Osx, OPN (Spp1), OCN (Bglap), and CyclinD1 normalized to Gapdh internal control using 2^-ΔΔCt^ method (n = 3). (F) Representative Western blot images showing expression of osteogenic proteins Runx2, Osx, OPN, OCN, CyclinD1, and GAPDH loading control. (G) Quantitative analysis of protein expression levels normalized to GAPDH (n = 3).
5). Activation of RXRα mitigates maternal separation-induced hippocampal neurodevelopmental impairment in mice by inhibiting oxidative stress and restoring mitochondrial homeostasis. Free radical biology & medicine, 2025 (PubMed: 41275931) [IF=7.1]
6). The critical role of ROS in Ermanin-induced melanogenesis. Free radical biology & medicine, 2021 (PubMed: 34560247) [IF=7.1]
7). CXCR4 influences PUFA desaturation and oxidative stress injury in experimental prostatitis mice by activating Fads2 via PPARγ. Free radical biology & medicine, 2024 (PubMed: 39094710) [IF=7.1]
8). A possible mechanism to the antidepressant-like effects of 20 (S)-protopanaxadiol based on its target protein 14-3-3 ζ. Journal of Ginseng Research, 2022 (PubMed: 36090685) [IF=6.8]

Application: WB    Species: Rat    Sample:

Fig. 5 Effects of fluoxetine (FLU) or PPD on the immobility time in the forced swimming test (FST) (A) and tail suspension test (TST) (B) in the corticosterone (CORT)-induced depression model. Representative Western blot images of GSK 3β, p-Ser9 GSK 3β, CREB, p-Ser133 CREB, BDNF, and β-actin expression levels (C). Western blot analysis showing relative protein levels of p-Ser133 CREB (D), p-Ser9 GSK 3β (E), and BDNF (F). ∗p < 0.05 vs. the control group, nsno statistical significance vs. the control group, #p < 0.05 vs. the CORT group.
9). β-Eudesmol in Rhizoma Atractylodis targets AVPR2 to inhibit the cAMP-AQP2 pathway and promote fluid metabolism. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 40618492) [IF=6.7]
10). Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics. PHYTOMEDICINE, 2021 (PubMed: 33360346) [IF=6.7]

Application: WB    Species: mice    Sample: hippocampus

Fig. 11. Effects of URE on 5-HT1A signaling pathway. (A) Western blotting analysis of 5-HT1A, BDNF, p-CREB, CREB, p-PKA, and PKA in hippocampus. (B-E) Quantitative data of 5-HT1A (B), BDNF (C), p-CREB/CREB (D), and p-PKA/PKA (E), data represent mean ± SEM (n = 3), ** p < 0.01, *** p < 0.001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the UCMS group. (F) The agonistic effect of URE against 5-HT1A receptor.
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