RHOBTB3 Antibody - #AF9183

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产品: RHOBTB3 抗体
货号: AF9183
描述: Rabbit polyclonal antibody to RHOBTB3
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Rat
预测: Pig, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 70kDa; 69kD(Calculated).
蛋白号: O94955
RRID: AB_2843373

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 50ul RMB¥ 1250 现货
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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Rat
预测:
Pig(100%), Horse(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
RHOBTB3 Antibody detects endogenous levels of total RHOBTB3.
RRID:
AB_2843373
引用格式: Affinity Biosciences Cat# AF9183, RRID:AB_2843373.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

KIAA0878; Rho related BTB domain containing 3; Rho related BTB domain containing protein 3; RHOBTB 3;

抗原和靶标

免疫原:
Uniprot:

O94955(RHBT3_HUMAN) >>Visit HPA database.

基因/基因ID:
RHOBTB3(22836)
表达:
O94955 RHBT3_HUMAN:

Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.

序列:
MSIHIVALGNEGDTFHQDNRPSGLIRTYLGRSPLVSGDESSLLLNAASTVARPVFTEYQASAFGNVKLVVHDCPVWDIFDSDWYTSRNLIGGADIIVIKYNVNDKFSFHEVKDNYIPVIKRALNSVPVIIAAVGTRQNEELPCTCPLCTSDRGSCVSTTEGIQLAKELGATYLELHSLDDFYIGKYFGGVLEYFMIQALNQKTSEKMKKRKMSNSFHGIRPPQLEQPEKMPVLKAEASHYNSDLNNLLFCCQCVDVVFYNPNLKKVVEAHKIVLCAVSHVFMLLFNVKSPTDIQDSSIIRTTQDLFAINRDTAFPGASHESSGNPPLRVIVKDALFCSCLSDILRFIYSGAFQWEELEEDIRKKLKDSGDVSNVIEKVKCILKTPGKINCLRNCKTYQARKPLWFYNTSLKFFLNKPMLADVVFEIQGTTVPAHRAILVARCEVMAAMFNGNYMEAKSVLIPVYGVSKETFLSFLEYLYTDSCCPAGIFQAMCLLICAEMYQVSRLQHICELFIITQLQSMPSRELASMNLDIVDLLKKAKFHHSDCLSTWLLHFIATNYLIFSQKPEFQDLSVEERSFVEKHRWPSNMYLKQLAEYRKYIHSRKCRCLVM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

细胞定位:

Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.

亚基结构:

Interacts with RAB9A and RAB9B (at lower level compared to RAB9A-binding). Interacts with M6PRBP1/TIP47.

蛋白家族:

Although predicted to be a GTP-binding protein because of the presence of a Rho-like region, binds and hydrolyzes ATP. In contrast to Rho-like proteins, the conserved Asp residue in position 138 in the G4 region is replaced by an Asn, decreasing the ability to bind GTP.

文献引用

1). MicroRNA-142-3p promotes renal cell carcinoma progression by targeting RhoBTB3 to regulate HIF-1 signaling and GGT/GSH pathways. Scientific reports, 2023 (PubMed: 37045834) [IF=3.8]

Application: WB    Species: Human    Sample: 786-O cells

Figure 4. miR-142-3p regulates RCC migration and invasion via hypoxia and GSH pathways by targeting RhoBTB3. 786-O cells were transfected with miR-control, miR-142-3p-mimics, and, miR-142-3p-inhibitors. Protein expression of RhoBTB3, hypoxia, and GSH pathway-related biomarkers, including HIF1A, VEGFA, and GGT1 using Western blot (A) (the original blots/gels are presented in Supplementary Fig. 3, the samples derive from the same experiment and that gels/blots were processed in parallel). The mRNA and protein expression of RhoBTB3, HIF1A, VEGFA, and GGT1 in 786-O cells was determined using qRT-PCR and Western blot, respectively (B,C). Expression of GGT1 differed significantly between RCC and the normal adjacent tissues (enhanced in KIRC, D, P 

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