产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9175, RRID:AB_2843365.
展开/折叠
Rab11 family interacting protein 4; RAB11 FIP4; Rab11 FIP4 like; RGD1305063; RP23-271B20.4;
抗原和靶标
Present at high level in testis (at protein level). Weakly expressed in other tissues.
- Q86YS3 RFIP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGGAGWSGAPAALLRSVRRLREVFEVCGRDPDGFLRVERVAALGLRFGQGEEVEKLVKYLDPNDLGRINFKDFCRGVFAMKGCEELLKDVLSVESAGTLPCAPEIPDCVEQGSEVTGPTFADGELIPREPGFFPEDEEEAMTLAPPEGPQELYTDSPMESTQSLEGSVGSPAEKDGGLGGLFLPEDKSLVHTPSMTTSDLSTHSTTSLISNEEQFEDYGEGDDVDCAPSSPCPDDETRTNVYSDLGSSVSSSAGQTPRKMRHVYNSELLDVYCSQCCKKINLLNDLEARLKNLKANSPNRKISSTAFGRQLMHSSNFSSSNGSTEDLFRDSIDSCDNDITEKVSFLEKKVTELENDSLTNGDLKSKLKQENTQLVHRVHELEEMVKDQETTAEQALEEEARRHREAYGKLEREKATEVELLNARVQQLEEENTELRTTVTRLKSQTEKLDEERQRMSDRLEDTSLRLKDEMDLYKRMMDKLRQNRLEFQKEREATQELIEDLRKELEHLQMYKLDCERPGRGRSASSGLGEFNARAREVELEHEVKRLKQENYKLRDQNDDLNGQILSLSLYEAKNLFAAQTKAQSLAAEIDTASRDELMEALKEQEEINFRLRQYMDKIILAILDHNPSILEIKH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86YS3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y60 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K505 | Ubiquitination | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot |
研究背景
Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. In case of infection by HCMV (human cytomegalovirus), may participate in egress of the virus out of nucleus; this function is independent of ARF6.
Endosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Recycling endosome membrane>Peripheral membrane protein. Cleavage furrow. Midbody. Cytoplasmic vesicle.
Note: Recruited to the cleavage furrow and the midbody during cytokinesis.
Present at high level in testis (at protein level). Weakly expressed in other tissues.
Homodimer. Forms a complex with Rab11 (RAB11A or RAB11B) and ARF6. Interacts with RAB11A; the interaction is direct. Forms a heterooligomeric complex with RAB11FIP2, RAB11FIP3 and RAB11FIP5. Interacts with ECPAS.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein gM/UL100.
The RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B).
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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