POLR3E Antibody - #AF9166

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产品: POLR3E 抗体
货号: AF9166
描述: Rabbit polyclonal antibody to POLR3E
应用: WB
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 80kDa; 80kD(Calculated).
蛋白号: Q9NVU0
RRID: AB_2843356

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(80%)
克隆:
Polyclonal
特异性:
POLR3E Antibody detects endogenous levels of total POLR3E.
RRID:
AB_2843356
引用格式: Affinity Biosciences Cat# AF9166, RRID:AB_2843356.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA directed RNA polymerase III 80 kDa polypeptide; DNA-directed RNA polymerase III subunit RPC5; FLJ10509; KIAA1452; polymerase (RNA) III (DNA directed) polypeptide E (80kD); Polymerase (RNA) III (DNA directed) polypeptide E; RNA polymerase III 80 kDa subunit RPC5; RNA polymerase III subunit C5; RPC5; SIN;

抗原和靶标

免疫原:
Uniprot:

Q9NVU0(RPC5_HUMAN) >>Visit HPA database.

基因/基因ID:
POLR3E(55718)
序列:
MANEEDDPVVQEIDVYLAKSLAEKLYLFQYPVRPASMTYDDIPHLSAKIKPKQQKVELEMAIDTLNPNYCRSKGEQIALNVDGACADETSTYSSKLMDKQTFCSSQTTSNTSRYAAALYRQGELHLTPLHGILQLRPSFSYLDKADAKHREREAANEAGDSSQDEAEDDVKQITVRFSRPESEQARQRRVQSYEFLQKKHAEEPWVHLHYYGLRDSRSEHERQYLLCPGSSGVENTELVKSPSEYLMMLMPPSQEEEKDKPVAPSNVLSMAQLRTLPLADQIKILMKNVKVMPFANLMSLLGPSIDSVAVLRGIQKVAMLVQGNWVVKSDILYPKDSSSPHSGVPAEVLCRGRDFVMWKFTQSRWVVRKEVATVTKLCAEDVKDFLEHMAVVRINKGWEFILPYDGEFIKKHPDVVQRQHMLWTGIQAKLEKVYNLVKETMPKKPDAQSGPAGLVCGDQRIQVAKTKAQQNHALLERELQRRKEQLRVPAVPPGVRIKEEPVSEEGEEDEEQEAEEEPMDTSPSGLHSKLANGLPLGRAAGTDSFNGHPPQGCASTPVARELKAFVEATFQRQFVLTLSELKRLFNLHLASLPPGHTLFSGISDRMLQDTVLAAGCKQILVPFPPQTAASPDEQKVFALWESGDMSDQHRQVLLEIFSKNYRVRRNMIQSRLTQECGEDLSKQEVDKVLKDCCVSYGGMWYLKGTVQS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
90
Dog
80
Xenopus
78
Zebrafish
78
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9NVU0 作为底物

Site PTM Type Enzyme
S36 Phosphorylation
K95 Acetylation
S161 Phosphorylation
S162 Phosphorylation
S192 Phosphorylation
Y224 Phosphorylation
T236 Phosphorylation
K287 Ubiquitination
K376 Ubiquitination
K383 Acetylation
K411 Ubiquitination
K432 Ubiquitination
Y434 Phosphorylation
K443 Ubiquitination
K465 Methylation
K467 Methylation
K467 Ubiquitination
K498 Sumoylation
S503 Phosphorylation
S522 Phosphorylation
S528 Phosphorylation
S544 Phosphorylation
S603 Phosphorylation
K659 Sumoylation
K682 Ubiquitination

研究背景

功能:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3D/RPC4.

研究领域

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

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