产品: 磷酸化 GATA1 (Ser142) 抗体
货号: AF3184
描述: Rabbit polyclonal antibody to Phospho-GATA1 (Ser142)
应用: WB IHC IF/ICC IP
反应: Human, Mouse, Rat
预测: Pig, Sheep, Rabbit
分子量: 43kDa; 43kD(Calculated).
蛋白号: P15976
RRID: AB_2834616

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Sheep(91%), Rabbit(100%)
克隆:
Polyclonal
特异性:
Phospho-GATA1 (Ser142) Antibody detects endogenous levels of GATA1 only when phosphorylated at Serine 142.
RRID:
AB_2834616
引用格式: Affinity Biosciences Cat# AF3184, RRID:AB_2834616.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Anemia, X-linked, without thrombocytopenia, included; ERYF 1; Eryf1; Erythroid transcription factor; Erythrold transcription factor 1; GATA 1; GATA binding factor 1; GATA binding protein 1 (globin transcription factor 1); GATA binding protein 1; GATA-1; GATA-binding factor 1; GATA1; GATA1_HUMAN; GF 1; GF-1; GF1; Globin transcription factor 1; NF E1; NF E1 DNA binding protein; NF-E1 DNA-binding protein; NFE 1; NFE1; Nuclear factor erythroid 1; Transcription factor GATA1; XLANP; XLTDA; XLTT;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P15976 GATA1_HUMAN:

Erythrocytes.

描述:
GATA1 Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms.
序列:
MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHQVNRPLTMRKDGIQTRNRKASGKGKKKRGSSLGGTGAAEGPAGGFMVVAGGSGSGNCGEVASGLTLGPPGTAHLYQGLGPVVLSGPVSHLMPFPGPLLGSPTGSFPTGPMPPTTSTTVVAPLSS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
100
Sheep
91
Horse
0
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P15976 作为底物

Site PTM Type Enzyme
S26 Phosphorylation P27361 (MAPK3)
S72 Phosphorylation
Y78 Phosphorylation
S91 Phosphorylation
S116 Phosphorylation
S131 Phosphorylation
K137 Sumoylation
S142 Phosphorylation
S161 Phosphorylation Q9P286 (PAK5)
S178 Phosphorylation
S187 Phosphorylation Q9P286 (PAK5)
Y223 Phosphorylation
Y231 Phosphorylation
K233 Acetylation
K245 Acetylation
K246 Acetylation
Y285 Phosphorylation
Y286 Phosphorylation
T304 Phosphorylation
S310 Phosphorylation P31749 (AKT1)
K312 Acetylation
K314 Acetylation
K315 Acetylation
K316 Acetylation
S319 Phosphorylation
S320 Phosphorylation
T324 Phosphorylation

研究背景

功能:

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.

翻译修饰:

Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).

Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity).

Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Erythrocytes.

亚基结构:

May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with BRD3 (By similarity). Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo (By similarity). Interacts with EP300. Interacts with MED1, CCAR1 and CALCOCO1. Interacts with CEBPE.

蛋白家族:

The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding (By similarity).

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