Nox3 Antibody - #AF9134

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产品: Nox3 抗体
货号: AF9134
描述: Rabbit polyclonal antibody to Nox3
应用: WB IHC
文献验证: WB
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 65kDa; 65kD(Calculated).
蛋白号: Q9HBY0
RRID: AB_2843324

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(80%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
Nox3 Antibody detects endogenous levels of total Nox3.
RRID:
AB_2843324
引用格式: Affinity Biosciences Cat# AF9134, RRID:AB_2843324.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 1.6.3.-; GP91 3; GP91-3; gp91phox homolog 3; Mitogenic oxidase 2; MOX 2; MOX-2; MOX2; NADPH oxidase 3; NADPH oxidase catalytic subunit-like 3; NOX 3; Nox3; NOX3_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q9HBY0(NOX3_HUMAN) >>Visit HPA database.

基因/基因ID:
NOX3(50508)
序列:
MMGCWILNEGLSTILVLSWLGINFYLFIDTFYWYEEEESFHYTRVILGSTLAWARASALCLNFNCMLILIPVSRNLISFIRGTSICCRGPWRRQLDKNLRFHKLVAYGIAVNATIHIVAHFFNLERYHWSQSEEAQGLLAALSKLGNTPNESYLNPVRTFPTNTTTELLRTIAGVTGLVISLALVLIMTSSTEFIRQASYELFWYTHHVFIVFFLSLAIHGTGRIVRGQTQDSLSLHNITFCRDRYAEWQTVAQCPVPQFSGKEPSAWKWILGPVVLYACERIIRFWRFQQEVVITKVVSHPSGVLELHMKKRGFKMAPGQYILVQCPAISSLEWHPFTLTSAPQEDFFSVHIRAAGDWTAALLEAFGAEGQALQEPWSLPRLAVDGPFGTALTDVFHYPVCVCVAAGIGVTPFAALLKSIWYKCSEAQTPLKLSKVYFYWICRDARAFEWFADLLLSLETRMSEQGKTHFLSYHIFLTGWDENQALHIALHWDENTDVITGLKQKTFYGRPNWNNEFKQIAYNHPSSSIGVFFCGPKALSRTLQKMCHLYSSADPRGVHFYYNKESF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
89
Dog
89
Chicken
89
Pig
80
Bovine
80
Sheep
80
Rabbit
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with and stabilizes CYBA/p22phox.

文献引用

1). Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway. Andrology, 2020 (PubMed: 32012485) [IF=3.2]

Application: WB    Species: rat    Sample: testis

FIGURE 3|BB attenuates ROS production in the testis of DM rats. (A) Representative Western blot results for NOX5, p22phox, gp91phox, NOX1, NOX3, and NOX4 in the testes of rats from all four groups.
2). Synergistic effect of elevated glucose levels with SARS-CoV-2 spike protein induced NOX-dependent ROS production in endothelial cells. Molecular Biology Reports, 2023 (PubMed: 37289363) [IF=2.6]

Application: WB    Species: Human    Sample: HUVECs

Fig. 1 Protein expression in different doses of S protein induced HUVECs. A. ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B. Relative expression of gray values for ACE2. C. Relative expression of gray values for TMPRSS2. D. NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E. Relative expression of gray values for NOX1. F. NOX2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G. Relative expression of gray values for NOX2. H. NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I. Relative expression of gray values for NOX3. J. NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K. Relative expression of gray values for NOX4

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