HC-II Antibody - #AF9084

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产品: HC-II 抗体
货号: AF9084
描述: Rabbit polyclonal antibody to HC-II
应用: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit, Dog
分子量: 57kDa; 57kD(Calculated).
蛋白号: P05546
RRID: AB_2843275

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(88%), Horse(100%), Rabbit(88%), Dog(100%)
克隆:
Polyclonal
特异性:
HC-II Antibody detects endogenous levels of total HC-II.
RRID:
AB_2843275
引用格式: Affinity Biosciences Cat# AF9084, RRID:AB_2843275.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

D22S673; HC II; HC-II; HC2; HCF2; HEP2_HUMAN; Heparin cofactor 2; Heparin cofactor II; HLS2; Leuserpin 2; LS2; Protease inhibitor leuserpin 2; Protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; SERPIND1; THPH10;

抗原和靶标

免疫原:
Uniprot:

P05546(HEP2_HUMAN) >>Visit HPA database.

基因/基因ID:
SERPIND1(3053)
表达:
P05546 HEP2_HUMAN:

Expressed predominantly in liver. Also present in plasma.

序列:
MKHSLNALLIFLIITSAWGGSKGPLDQLEKGGETAQSADPQWEQLNNKNLSMPLLPADFHKENTVTNDWIPEGEEDDDYLDLEKIFSEDDDYIDIVDSLSVSPTDSDVSAGNILQLFHGKSRIQRLNILNAKFAFNLYRVLKDQVNTFDNIFIAPVGISTAMGMISLGLKGETHEQVHSILHFKDFVNASSKYEITTIHNLFRKLTHRLFRRNFGYTLRSVNDLYIQKQFPILLDFKTKVREYYFAEAQIADFSDPAFISKTNNHIMKLTKGLIKDALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNEREVVKVSMMQTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTLEAQLTPRVVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFDKNGNMAGISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRFTVDRPFLFLIYEHRTSCLLFMGRVANPSRS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Bovine
88
Rabbit
88
Zebrafish
71
Sheep
63
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P05546 作为底物

Site PTM Type Enzyme
S37 Phosphorylation
N49 N-Glycosylation
T66 Phosphorylation
Y79 Phosphorylation
Y138 Phosphorylation
N188 N-Glycosylation
N387 N-Glycosylation

研究背景

功能:

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

翻译修饰:

Phosphorylated by FAM20C in the extracellular medium.

组织特异性:

Expressed predominantly in liver. Also present in plasma.

蛋白家族:

The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.

Belongs to the serpin family.

研究领域

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

文献引用

1). Genistein treatment duration effects biomarkers of cell motility in human prostate. PLoS One (PubMed: 30917169) [IF=3.7]

Application: WB    Species: human    Sample: human prostate cell

S5 Fig. | Effect of BASP1 and HCF2 knockdown and overexpression on respective transcript and protein levels.E. comparison of BASP1 and HCF2 protein expression in native cell lines measured by Western blot.

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