Phospho-Ezrin (Tyr478) Antibody - #AF3174
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3174, RRID:AB_2834606.
展开/折叠
Villin 2 ezrin; CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; Epididymis secretory protein Li 105; EZR; EZRI_HUMAN; Ezrin; FLJ26216; HEL S 105; MGC1584; p81; VIL 2; VIL2; Villin 2 (ezrin); Villin 2; Villin-2; Villin2;
抗原和靶标
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
- P15311 EZRI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15311 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K3 | Ubiquitination | Uniprot | |
K35 | Acetylation | Uniprot | |
K35 | Ubiquitination | Uniprot | |
T57 | Phosphorylation | Uniprot | |
K60 | Acetylation | Uniprot | |
K64 | Ubiquitination | Uniprot | |
S66 | Phosphorylation | P17612 (PRKACA) | Uniprot |
K72 | Ubiquitination | Uniprot | |
K79 | Acetylation | Uniprot | |
K79 | Ubiquitination | Uniprot | |
K83 | Ubiquitination | Uniprot | |
K107 | Ubiquitination | Uniprot | |
S112 | Phosphorylation | Uniprot | |
Y116 | Phosphorylation | Uniprot | |
Y137 | Phosphorylation | Uniprot | |
K139 | Acetylation | Uniprot | |
K139 | Ubiquitination | Uniprot | |
K143 | Ubiquitination | Uniprot | |
S144 | Phosphorylation | Uniprot | |
Y146 | Phosphorylation | P00533 (EGFR) , P16591 (FER) , P12931 (SRC) , P06239 (LCK) | Uniprot |
S149 | Phosphorylation | Uniprot | |
K162 | Ubiquitination | Uniprot | |
R171 | Methylation | Uniprot | |
K184 | Acetylation | Uniprot | |
K184 | Ubiquitination | Uniprot | |
Y191 | Phosphorylation | Uniprot | |
Y201 | Phosphorylation | Uniprot | |
Y205 | Phosphorylation | Uniprot | |
K209 | Ubiquitination | Uniprot | |
K211 | Ubiquitination | Uniprot | |
K230 | Sumoylation | Uniprot | |
K230 | Ubiquitination | Uniprot | |
K233 | Sumoylation | Uniprot | |
K233 | Ubiquitination | Uniprot | |
T235 | Phosphorylation | Q00535 (CDK5) | Uniprot |
K237 | Ubiquitination | Uniprot | |
S243 | Phosphorylation | Uniprot | |
S249 | Phosphorylation | Uniprot | |
K253 | Acetylation | Uniprot | |
K253 | Ubiquitination | Uniprot | |
K254 | Ubiquitination | Uniprot | |
K258 | Acetylation | Uniprot | |
K258 | Ubiquitination | Uniprot | |
K262 | Acetylation | Uniprot | |
K262 | Sumoylation | Uniprot | |
K262 | Ubiquitination | Uniprot | |
K263 | Acetylation | Uniprot | |
K263 | Ubiquitination | Uniprot | |
Y270 | Phosphorylation | Uniprot | |
R273 | Methylation | Uniprot | |
Y291 | Phosphorylation | Uniprot | |
K296 | Ubiquitination | Uniprot | |
T299 | Phosphorylation | Uniprot | |
K306 | Methylation | Uniprot | |
K306 | Ubiquitination | Uniprot | |
T332 | Phosphorylation | Uniprot | |
K337 | Ubiquitination | Uniprot | |
K344 | Ubiquitination | Uniprot | |
Y354 | Phosphorylation | P16591 (FER) , P00533 (EGFR) | Uniprot |
K357 | Sumoylation | Uniprot | |
K357 | Ubiquitination | Uniprot | |
S366 | Phosphorylation | Uniprot | |
R393 | Methylation | Uniprot | |
K412 | Acetylation | Uniprot | |
K412 | Ubiquitination | Uniprot | |
S413 | Phosphorylation | Uniprot | |
Y424 | Phosphorylation | Uniprot | |
T425 | Phosphorylation | Uniprot | |
K427 | Ubiquitination | Uniprot | |
R435 | Methylation | Uniprot | |
K438 | Ubiquitination | Uniprot | |
K450 | Ubiquitination | Uniprot | |
K458 | Ubiquitination | Uniprot | |
T468 | Phosphorylation | Uniprot | |
Y478 | Phosphorylation | P12931 (SRC) , P16591 (FER) | Uniprot |
S482 | Phosphorylation | Uniprot | |
Y483 | Phosphorylation | Uniprot | |
S488 | Phosphorylation | Uniprot | |
T497 | Phosphorylation | Uniprot | |
Y499 | Phosphorylation | Uniprot | |
K523 | Ubiquitination | Uniprot | |
S535 | Phosphorylation | Uniprot | |
S536 | Phosphorylation | Uniprot | |
S539 | Phosphorylation | Uniprot | |
T548 | Phosphorylation | Uniprot | |
Y565 | Phosphorylation | Uniprot | |
T567 | Phosphorylation | P17252 (PRKCA) , Q5S007 (LRRK2) , P41279 (MAP3K8) , O94804 (STK10) , O95819 (MAP4K4) , P25098 (GRK2) , P31751 (AKT2) , Q13464 (ROCK1) , Q04759 (PRKCQ) , Q9P289 (STK26) , P41743 (PRKCI) , O75116 (ROCK2) | Uniprot |
T576 | Phosphorylation | Uniprot |
研究背景
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Apical cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection. Cell projection>Microvillus membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cell projection>Microvillus.
Note: Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein (cytoplasmic side).
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS. Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites. Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX (By similarity). Interacts with PDPN (via cytoplasmic domain); activates RHOA and promotes epithelial-mesenchymal transition. Interacts with SPN, CD44 and ICAM2 (By similarity).
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Digestive system > Gastric acid secretion.
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.