CaMKII beta/gamma Antibody - #AF9030

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产品: CaMKII beta/gamma 抗体
货号: AF9030
描述: Rabbit polyclonal antibody to CaMKII beta/gamma
应用: WB IF/ICC
反应: Human, Mouse, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 62kDa; 63kD,73kD(Calculated).
蛋白号: Q13555 | Q13554
RRID: AB_2843221

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(90%)
克隆:
Polyclonal
特异性:
CaMKII beta/gamma Antibody detects endogenous levels of total CaMKII beta/gamma.
RRID:
AB_2843221
引用格式: Affinity Biosciences Cat# AF9030, RRID:AB_2843221.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CaM kinase II; CaM kinase II gamma chain; CaM kinase II subunit gamma; CAMK; CAMK II; CaMK II subunit gamma; CAMK2G; CAMKG; CAMKII; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit alpha; CaM kinase II subunit delta; CAM2; CaMK-II subunit alpha; CaMK-II subunit delta; CAMK2A; CAMK2B; CAMKA; CAMKB; CAMKD; CaMKII alpha; KIAA0968;

抗原和靶标

免疫原:
Uniprot:

Q13555(KCC2G_HUMAN) >>Visit HPA database.

Q13554(KCC2B_HUMAN) >>Visit HPA database.

基因/基因ID:
CAMK2G(818) | CAMK2B(816)
表达:
Q13555 KCC2G_HUMAN:

Expressed in skeletal muscle.

Q13554 KCC2B_HUMAN:

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

序列:
MATTATCTRFTDDYQLFEELGKGAFSVVRRCVKKTSTQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIHQILESVNHIHQHDIVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSAAKSLLNKKSDGGVKKRKSSSSVHLMPQSNNKNSLVSPAQEPAPLQTAMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKGRVPEGRSSRDRTAPSAGMQPQPSLCSSAMRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAPAAPLQ

MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKAPRVPDILSSVRRGSGAPEAEGPLPCPSPAPFSPLPAPSPRISDILNSVRRGSGTPEAEGPLSAGPPPCLSPALLGPLSSPSPRISDILNSVRRGSGTPEAEGPSPVGPPPCPSPTIPGPLPTPSRKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Chicken
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13555/Q13554 作为底物

Site PTM Type Enzyme
K57 Ubiquitination
S79 Phosphorylation
K138 Ubiquitination
S146 Phosphorylation
K227 Acetylation
K227 Ubiquitination
Y231 Phosphorylation
S235 Phosphorylation
K246 Ubiquitination
T254 Phosphorylation
K259 Ubiquitination
K268 Ubiquitination
S276 Phosphorylation
S280 Phosphorylation
T287 Phosphorylation Q13554 (CAMK2B)
K299 Methylation
K301 Methylation
T306 Phosphorylation
T307 Phosphorylation
T311 Phosphorylation
S315 Phosphorylation
T328 Phosphorylation
K342 Acetylation
S394 Phosphorylation
S395 Phosphorylation
S397 Phosphorylation
T400 Phosphorylation
T401 Phosphorylation
S423 Phosphorylation
S436 Phosphorylation
S441 Phosphorylation
S456 Phosphorylation
S461 Phosphorylation
T463 Phosphorylation
S471 Phosphorylation
S479 Phosphorylation
S499 Phosphorylation
S504 Phosphorylation
T506 Phosphorylation
S522 Phosphorylation
Site PTM Type Enzyme
Y14 Phosphorylation
K22 Ubiquitination
S26 Phosphorylation
K34 Ubiquitination
T35 Phosphorylation
K43 Ubiquitination
K57 Ubiquitination
K69 Ubiquitination
S79 Phosphorylation
S114 Phosphorylation
K138 Ubiquitination
S146 Phosphorylation
K227 Acetylation
K227 Ubiquitination
Y231 Phosphorylation
S235 Phosphorylation
K246 Ubiquitination
T254 Phosphorylation
K259 Ubiquitination
K268 Ubiquitination
C273 S-Nitrosylation
S276 Phosphorylation
S280 Phosphorylation Q13555 (CAMK2G)
T287 Phosphorylation Q13555 (CAMK2G)
K299 Methylation
K301 Methylation
T306 Phosphorylation
T307 Phosphorylation
S311 Phosphorylation
S315 Phosphorylation
S319 Phosphorylation Q13555 (CAMK2G)
S325 Phosphorylation
S334 Phosphorylation
S336 Phosphorylation
S349 Phosphorylation
S352 Phosphorylation
T382 Phosphorylation
S384 Phosphorylation
T388 Phosphorylation
S419 Phosphorylation
S484 Phosphorylation

翻译修饰 - Q13555/Q13554 作为激酶

Substrate Site Source
O14490-2 (DLGAP1) S349 Uniprot
O14490-2 (DLGAP1) S393 Uniprot
P14921-1 (ETS1) S251 Uniprot
P14921-1 (ETS1) S257 Uniprot
P14921-1 (ETS1) S282 Uniprot
P14921-1 (ETS1) S285 Uniprot
P16949 (STMN1) S16 Uniprot
P26678 (PLN) T17 Uniprot
P35222 (CTNNB1) T332 Uniprot
P35222 (CTNNB1) T472 Uniprot
P35222 (CTNNB1) S552 Uniprot
P42224 (STAT1) S727 Uniprot
P49840 (GSK3A) S21 Uniprot
Q01970-1 (PLCB3) S537 Uniprot
Q12879-1 (GRIN2A) S1291 Uniprot
Q13131 (PRKAA1) T183 Uniprot
Q13224 (GRIN2B) S383 Uniprot
Q13224 (GRIN2B) S1303 Uniprot
Q13554-1 (CAMK2B) T287 Uniprot
Q9NQC7 (CYLD) S362 Uniprot
Q9NQC7 (CYLD) S418 Uniprot
Q9NQC7 (CYLD) S772 Uniprot
Substrate Site Source
O60266-1 (ADCY3) S1076 Uniprot
P00533-1 (EGFR) S1070 Uniprot
P00533-1 (EGFR) S1071 Uniprot
P07101-1 (TH) S19 Uniprot
P10276 (RARA) T210 Uniprot
P17600-2 (SYN1) S568 Uniprot
P17600-2 (SYN1) S605 Uniprot
P18846-1 (ATF1) S63 Uniprot
P21333-2 (FLNA) S2515 Uniprot
P21817-1 (RYR1) S2843 Uniprot
P28329 (CHAT) T574 Uniprot
P35270 (SPR) S213 Uniprot
P40763 (STAT3) S727 Uniprot
P42224-1 (STAT1) S727 Uniprot
P42261-1 (GRIA1) S645 Uniprot
P42261-1 (GRIA1) S849 Uniprot
P48058 (GRIA4) S862 Uniprot
P55042 (RRAD) S273 Uniprot
Q01970-1 (PLCB3) S537 Uniprot
Q03060-16 (CREM) S87 Uniprot
Q13555 (CAMK2G) S280 Uniprot
Q13555 (CAMK2G) T287 Uniprot
Q13555 (CAMK2G) S319 Uniprot
Q15121-1 (PEA15) S116 Uniprot
Q15746-1 (MYLK) S1760 Uniprot
Q92934 (BAD) S134 Uniprot
Q9UQL6 (HDAC5) S259 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot

研究背景

功能:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In the central nervous system, it is involved in the regulation of neurite formation and arborization. It may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning.

翻译修饰:

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

细胞定位:

Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in skeletal muscle.

亚基结构:

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.

蛋白家族:

The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

功能:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

翻译修饰:

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

细胞定位:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse.
Note: In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

亚基结构:

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other Ref.19). Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ. Interacts with FOXO3 (By similarity). Interacts (when in a kinase inactive state not associated with calmodulin) with ARC; leading to target ARC to inactive synapses (By similarity).

蛋白家族:

The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

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