MCM5 Antibody - #AF0207

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产品: MCM5 抗体
货号: AF0207
描述: Rabbit polyclonal antibody to MCM5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 90kDa; 82kD(Calculated).
蛋白号: P33992
RRID: AB_2833394

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
MCM5 Antibody detects endogenous levels of total MCM5.
RRID:
AB_2833394
引用格式: Affinity Biosciences Cat# AF0207, RRID:AB_2833394.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl,sodium azide and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CDC 46; CDC46; CDC46 homolog; Cell division cycle 46; DNA replication licensing factor; DNA replication licensing factor MCM 5; DNA replication licensing factor MCM5; MCM 5; MCM5; MCM5_HUMAN; MGC5315; Minichromosome maintenance complex component 5; Minichromosome maintenance deficient (S. cerevisiae) 5; Minichromosome maintenance deficient 5; Minichromosome maintenance deficient protein 5; P1 CDC46; P1-CDC46;

抗原和靶标

免疫原:
Uniprot:

P33992(MCM5_HUMAN) >>Visit HPA database.

基因/基因ID:
MCM5(4174)
描述:
MCM5 Belongs to the MCM family
序列:
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEVLQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLTNIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQVDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
92
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P33992 作为底物

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S12 Phosphorylation
S14 Phosphorylation
K36 Ubiquitination
K54 Ubiquitination
K129 Ubiquitination
S130 Phosphorylation
S133 Phosphorylation
K141 Ubiquitination
K150 Ubiquitination
S159 Phosphorylation
K206 Ubiquitination
Y212 Phosphorylation
K218 Ubiquitination
K220 Acetylation
K220 Ubiquitination
K228 Ubiquitination
Y248 Phosphorylation
R251 Methylation
K256 Ubiquitination
Y269 Phosphorylation
K273 Ubiquitination
S279 Phosphorylation
R280 Methylation
T303 Phosphorylation
S315 Phosphorylation
Y332 Phosphorylation
S336 Phosphorylation
K337 Ubiquitination
S338 Phosphorylation
S342 Phosphorylation
K350 Ubiquitination
K351 Ubiquitination
S361 Phosphorylation
K363 Ubiquitination
T385 Phosphorylation
K387 Acetylation
K387 Ubiquitination
K392 Acetylation
K392 Ubiquitination
K396 Acetylation
K396 Ubiquitination
Y403 Phosphorylation
K407 Ubiquitination
S423 Phosphorylation
S424 Phosphorylation
K471 Ubiquitination
C482 S-Nitrosylation
K499 Ubiquitination
R528 Methylation
K561 Ubiquitination
K581 Acetylation
K581 Ubiquitination
K583 Ubiquitination
S604 Phosphorylation
S605 Phosphorylation
K625 Ubiquitination
K627 Ubiquitination
T633 Phosphorylation O14757 (CHEK1)
S693 Phosphorylation
K696 Acetylation
K696 Ubiquitination
K702 Ubiquitination

研究背景

功能:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.

细胞定位:

Nucleus. Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with ANKRD17.

蛋白家族:

Belongs to the MCM family.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

文献引用

1). Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis. International Journal of Molecular Sciences, 2023 (PubMed: 37047268) [IF=5.6]

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