产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9018, RRID:AB_2843209.
展开/折叠
ARRD4_HUMAN; ARRDC 4; Arrdc4; Arrestin domain containing protein 4; Arrestin domain-containing protein 4; FLJ36045;
抗原和靶标
- Q8NCT1 ARRD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGGEAGCAAAVGAEGRVKSLGLVFEDERKGCYSSGETVAGHVLLEASEPVALRALRLEAQGRATAAWGPSTCPRASASTAALAVFSEVEYLNVRLSLREPPAGEGIILLQPGKHEFPFRFQLPSEPLVTSFTGKYGSIQYCVRAVLERPKVPDQSVKRELQVVSHVDVNTPALLTPVLKTQEKMVGCWFFTSGPVSLSAKIERKGYCNGEAIPIYAEIENCSSRLIVPKAAIFQTQTYLASGKTKTIRHMVANVRGNHIASGSTDTWNGKTLKIPPVTPSILDCCIIRVDYSLAVYIHIPGAKKLMLELPLVIGTIPYNGFGSRNSSIASQFSMDMSWLTLTLPEQPEAPPNYADVVSEEEFSRHIPPYPQPPNCEGEVCCPVFACIQEFRFQPPPLYSEVDPHPSDVEESQPVSFIL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8NCT1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T37 | Phosphorylation | Uniprot | |
| Y206 | Phosphorylation | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| Y398 | Phosphorylation | Uniprot |
研究背景
Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates (By similarity). Plays a role in endocytosis of activated G protein-coupled receptors (GPCRs) (Probable). Through an ubiquitination-dependent mechanism plays also a role in the incorporation of SLC11A2 into extracellular vesicles (By similarity). May play a role in glucose uptake.
Early endosome. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle.
Note: Also found in extracellular vesicles different from exosomes.
Interacts with ADRB2. Interacts (via PPxY motifs) with ITCH, NEDD4L and WWP2. Interacts with AVPR2. Identified in a complex containing at least ARRDC4, AVPR2 and HGS. Interacts with SLC11A2; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism (By similarity).
Belongs to the arrestin family.
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