产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9016, RRID:AB_2843207.
展开/折叠
ARRD1_HUMAN; ARRDC 1; ARRDC1; Arrestin domain containing 1; Arrestin domain containing protein 1; Arrestin domain-containing protein 1; MGC40555; OTTHUMP00000022707; OTTHUMP00000022709; RP11-48C7.5;
抗原和靶标
- Q8N5I2 ARRD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGRVQLFEISLSHGRVVYSPGEPLAGTVRVRLGAPLPFRAIRVTCIGSCGVSNKANDTAWVVEEGYFNSSLSLADKGSLPAGEHSFPFQFLLPATAPTSFEGPFGKIVHQVRAAIHTPRFSKDHKCSLVFYILSPLNLNSIPDIEQPNVASATKKFSYKLVKTGSVVLTASTDLRGYVVGQALQLHADVENQSGKDTSPVVASLLQKVSYKAKRWIHDVRTIAEVEGAGVKAWRRAQWHEQILVPALPQSALPGCSLIHIDYYLQVSLKAPEATVTLPVFIGNIAVNHAPVSPRPGLGLPPGAPPLVVPSAPPQEEAEAEAAAGGPHFLDPVFLSTKSHSQRQPLLATLSSVPGAPEPCPQDGSPASHPLHPPLCISTGATVPYFAEGSGGPVPTTSTLILPPEYSSWGYPYEAPPSYEQSCGGVEPSLTPES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates. Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles. More generally, plays a role in the extracellular transport of proteins between cells through the release in the extracellular space of microvesicles. By participating to the ITCH-mediated ubiquitination and subsequent degradation of NOTCH1, negatively regulates the NOTCH signaling pathway.
Ubiquitinated. Ubiquitination by WWP2; promotes localization to extracellular microvesicles. Ubiquitinated by WWP1.
Cell membrane.
Note: Also found in extracellular vesicles different from exosomes.
Interacts (via PPxY motifs) with ITCH (via WW domains); the interaction is direct and participates to the recruitment of the ubiquitin-protein ligase ITCH to the NOTCH1 receptor. Interacts with ARRB1 and ARRB2; the interaction is direct. Interacts with TSG101; may recruit TSG101 to the plasma membrane. Interacts (via PPxY motifs) with WWP2 (via WW domains); ubiquitinates ARRDC1. Interacts with SLC11A2; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism. Interacts with WWP1 (via WW domains). Interacts with NEDD4 (via WW domains). Interacts with ALIX.
The PPxY motifs mediate interaction with WW domain-containing ubiquitin-protein ligases.
Belongs to the arrestin family.
限制条款
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