OTUB1 Antibody - #DF9998

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产品: OTUB1 抗体
货号: DF9998
描述: Rabbit polyclonal antibody to OTUB1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken
蛋白号: Q96FW1
RRID: AB_2843190

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
OTUB1 Antibody detects endogenous levels of total OTUB1.
RRID:
AB_2843190
引用格式: Affinity Biosciences Cat# DF9998, RRID:AB_2843190.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Deubiquitinating enzyme OTUB1; hOTU1; HSPC263; OTB1; OTU domain containing ubiquitin aldehyde binding protein 1; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing Ubal-binding protein 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; OTU1; Otub1; OTUB1_HUMAN; Otubain 1; Otubain-1; Ubiquitin specific processing protease OTUB1; Ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; Ubiquitin-specific-processing protease OTUB1;

抗原和靶标

免疫原:

A synthesized peptide derived from human OTUB1, corresponding to a region within the internal amino acids.

基因/基因ID:
OTUB1(55611)

文献引用

1). Target deubiquitinase OTUB1 as a therapeatic strategy for BLCA via β-catenin/necroptosis signal pathway. International journal of biological sciences, 2024 (PubMed: 39113709) [IF=8.2]

Application: WB    Species: Mouse    Sample: bladder tissues

Figure 1. OTUB1 is elevated in BLCA and related to poor prognosis. A. Volcano plot of differentially expressed genes (DEGs) in TCGA BLCA data. B. The heatmap showed the expression of all members in the OTU deubiquitinase superfamily in BLCA and para-cancer tissues. C. Box plots of the relative mRNA expression levels of OTUB1 in BLCA and para-cancer samples from the TCGA database. D, E. The analysis of OTUB1 protein and mRNA expression in BLCA (T) and paired normal bladder (N) tissues by western blot and qRT- PCR. F. Representative immunohistochemical staining of OTUB1 and ki-67 in BLCA and paired normal bladder tissues (100X, 200X). G. Kaplan-Meier plots representing survival probabilities in 42 BLCA patients according to the relative expression level of OTUB1. Statistical analysis was conducted using the Student t-test and log-rank test.

Application: IHC    Species: Mouse    Sample: bladder tissues

Figure 1. OTUB1 is elevated in BLCA and related to poor prognosis. A. Volcano plot of differentially expressed genes (DEGs) in TCGA BLCA data. B. The heatmap showed the expression of all members in the OTU deubiquitinase superfamily in BLCA and para-cancer tissues. C. Box plots of the relative mRNA expression levels of OTUB1 in BLCA and para-cancer samples from the TCGA database. D, E. The analysis of OTUB1 protein and mRNA expression in BLCA (T) and paired normal bladder (N) tissues by western blot and qRT- PCR. F. Representative immunohistochemical staining of OTUB1 and ki-67 in BLCA and paired normal bladder tissues (100X, 200X). G. Kaplan-Meier plots representing survival probabilities in 42 BLCA patients according to the relative expression level of OTUB1. Statistical analysis was conducted using the Student t-test and log-rank test.
2). OTUB1 promotes progression and proliferation of prostate cancer via deubiquitinating and stabling cyclin E1. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 33537306) [IF=4.6]

Application: WB    Species: Mice    Sample: PC3 and C4-2 cells

Figure 4 OTUB1 promotes and regulates the expression of Cyclin E1. (A) The relationship between OTUB1 and Cyclin E1 was analyzed via gene MANIA database. (B) The protein expression of Cyclin E1 in BPH, ADPC, and CRPC was detected by IHC staining. (C) Western blotting detecting the expression of Cyclin E1 in PC3 and C4-2 cells transfected with otub1 overexpression and otub1 c91s. (D) Western blotting detecting the expression of Cyclin E1 in PC3 and C4-2 cells transfected with OTUB1 siRNA. (E) Western blotting detecting the expression of Cyclin E1 in PC3 and C4-2 cells transfected with an increasing gradient OTUB1.

Application: IHC    Species: Mice    Sample: tumors

Figure 7 OTUB1 promotes the growth of PC3 cell via increasing Cyclin E1 in vivo. (A,B) PC3 cells transfected with OTUB1 overexpression and otub1 c91s were transplanted subcutaneously in nude mice. The effect of otub1 overexpression, otub1 c91s, and RO-3306 on the growth of PCa. (C) The tumor volume was measured with a caliper. (D) The tumor volumes were measured daily when the diameter grew into 2 mm. (E) IHC staining detecting the expression of OTUB1, ki-67, and Cyclin E1 in these tumors from nude mice. ****P < 0.0001.

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