Phospho-NCF1/p47-phox (Ser359) Antibody - #AF3167

Figure S6 Oxidized rGO increased lipid peroxidation and NOX2 activation in PC12 cells. Oxidized rGO (c-rGO) was collected after pristine rGO (p-rGO) was cultured with PC12 cells for 3 h. (A-B) PC12 cells were pretreated with NAC for 30 min before being treated with two kinds of rGO (20 µg/mL) for 1 h and stained with BODIPY 581/591 C11 reagent. The lipid peroxidation degrees were quantified via FITC fluorescence intensity, with n = 10 fields per experimental condition from 3 independent tests. Scale bar: 100 μm. (C-D) After PC12 cells were treated as described in (A), plasma membrane proteins were isolated to detect the expression levels of NOX2, p-p47phox, p-p67phox and Rac2. The protein levels were quantified after normalization to ATPase. The results represent the mean ± SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001 compared with the control group and with the p-rGO group.

Figure 5 TZG significantly regulated the expression of NETs formation-related indicators, and the corresponding proteins in PI3K-RAC2 signaling (A–E). Serum levels of dsDNA, IL-8, TNF-α, CitH3, and MPO detected by ELISA analyses, respectively. Samples in (A)∼(E) were obtained from the blood supernatant of rats in different groups. (F–I) The ratios of p-PI3K/PI3K and p-P47/P47, and the expression levels of RAC2 and PAD4 proteins detected by western blot analyses, respectively. Samples in (F)∼(I) were obtained from MTrPs tissues of rats in different groups. The number of rats in each group was 8. Data are expressed as the mean ± S.D. “∗,” “∗∗,” and “∗∗∗,” p < 0.05, p < 0.01, and p < 0.001, respectively, comparison with the normal control group. “#,” “##,” and “###,” p < 0.05, p < 0.01, and p < 0.001, respectively, comparison with the MPS model group.