ZNF280B Antibody - #DF9950

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产品: ZNF280B 抗体
货号: DF9950
描述: Rabbit polyclonal antibody to ZNF280B
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 62 kDa; 62kD(Calculated).
蛋白号: Q86YH2
RRID: AB_2843144

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
ZNF280B Antibody detects endogenous levels of total ZNF280B.
RRID:
AB_2843144
引用格式: Affinity Biosciences Cat# DF9950, RRID:AB_2843144.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5''OY11.1; D87009.C22.3; SUHW2; Suppressor of hairy wing homolog 2; Z280B_HUMAN; Zinc finger protein 279; Zinc finger protein 280B; Zinc finger protein 632; ZNF279; ZNF280B; ZNF632;

抗原和靶标

免疫原:
Uniprot:

Q86YH2(Z280B_HUMAN) >>Visit HPA database.

基因/基因ID:
ZNF280B(140883)
序列:
MEQSCEEEKEPEPQKNIQETKQVDDEDAELIFVGVEHVNEDAELIFVGVTSNSKPVVSNILNRVTPGSWSRRKKYDHLRKDTARKLQPKSHETVTSEAVTVLPASQLESRSTDSPIIIEPLSKPDYRNSSPQVVPNNSSELPSPLITFTDSLHHPVSTALSVGGINESPRVSKQLSTFEVNSINPKRAKLRDGIIEGNSSASFPSDTFHTMNTQQSTPSNNVHTSLSHVQNGAPFPAAFPKDNIHFKPINTNLDRENELAKTDILSLTSQNKTFDPKKENPIVLLSDFYYGQHKGEGQPEQKTHTTFKCLSCVKVLKNVKFMNHVKHHLEFEKQRNDSWENHTTCQHCHRQFPTPFQLQCHIENVHTAQEPSTVCKICELSFETDQVLLQHMKDHHKPGEMPYVCQVCHYRSSVFADVETHFRTCHENTKNLLCPFCLKIFKTATPYMCHYRGHWGKSAHQCSKCRLQFLTFKEKMEHKTQCHQMFKKPKQLEGLPPETKVTIQVSLEPLQPGSVDVASITVSTSDSEPSLPRSKSKISKKSH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Bovine
90
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

May function as a transcription factor.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

文献引用

1). microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma. ONCOLOGY REPORTS, 2021 (PubMed: 33416178) [IF=3.8]

Application: WB    Species: Human    Sample: DLBCL cells

Figure 4. - Prediction and identification of miR-196a-3p target genes in DLBCL cells. (A) miRNA targets were predicted using TargetScan, microRNA, miRDB and DIANA-microT. (B) The protein levels of 4 miR-196a-3p candidate target genes were analyzed by western blot analysis. Up, protein bands; down, grey value. (C) mRNA levels of 4 miR-196a-3p candidate target genes were analyzed by RT-qPCR. (D) 293T cells were transiently transfected with Renilla luciferase reporter vectors containing either wild-type or mutant ARF4 3′-UTR seed sequence, and luciferase activity was measured following co-transfection with miR-196a-3p or NC. *P

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