产品: NHE-3 抗体
货号: DF9937
描述: Rabbit polyclonal antibody to NHE-3
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P48764
RRID: AB_2843131

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 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
NHE-3 Antibody detects endogenous levels of total NHE-3.
RRID:
AB_2843131
引用格式: Affinity Biosciences Cat# DF9937, RRID:AB_2843131.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

MGC126718; MGC126720; Na(+)/H(+) exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3; Solute carrier family 9 (sodium/hydrogen exchanger), member 3; Solute carrier family 9 member 3;

抗原和靶标

免疫原:

A synthesized peptide derived from human NHE-3, corresponding to a region within the internal amino acids.

基因/基因ID:

研究领域

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Protein digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection. Frontiers in Microbiology, 2018 (PubMed: 30483239) [IF=5.2]

Application: WB    Species: pig    Sample: IPEC-J2 cells

FIGURE 1 | The effect of TGEV on Na+ concentration and the relative expression of NHE3 in IPEC-J2 cells. (A,B) Intra and extracellular Na+ concentration in cells infected with TGEV at 0, 48, and 72 h post infection. (C) NHE3 mRNA expression levels were detected using qRT-PCR and normalized by the β-actin mRNA level.(D)NHE3 protein levels were analyzed by Western blotting.

2). Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway. HYPERTENSION RESEARCH, 2021 (PubMed: 32908237) [IF=4.3]

Application: WB    Species: rat    Sample: renal cortex and medulla

Fig. 4 |Effects of NaBu on DOCA/salt-induced renal sodium transporter proteins.Representative immunoblots of α-ENaC, β-ENaC, γ-ENaC,NHE3, NCC, and NKCC-2 and summarized intensities of blots in the renal cortex (a) and medulla (b) (n = 6–7 per group).αENaC α-subunit of epithelial sodium channel, β-ENaC βsubunit of epithelial sodium channel, γ-ENaC γ-subunit of epithelial sodium channel,NHE3 sodium/proton exchanger isoform 3, NKCC-2 Na-K-2Cl cotransporter, NCC Na-Cl Cotransporter

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