Phospho-SRF (Ser99)[Ser103] Antibody - #AF3160
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3160, RRID:AB_2834595.
展开/折叠
c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN;
抗原和靶标
- P11831 SRF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P11831 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | Phosphorylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| R37 | Methylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| Y76 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S79 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S83 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S85 | Phosphorylation | P49137 (MAPKAPK2) , P68400 (CSNK2A1) | Uniprot |
| S101 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Q15418 (RPS6KA1) , O75582 (RPS6KA5) , Q9UQM7 (CAMK2A) , P49137 (MAPKAPK2) | Uniprot |
| K147 | Sumoylation | Uniprot | |
| T159 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) , Q09013 (DMPK) , P17252 (PRKCA) | Uniprot |
| T160 | Phosphorylation | Q9UQM7 (CAMK2A) , Q05655 (PRKCD) | Uniprot |
| S162 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S277 | O-Glycosylation | Uniprot | |
| S307 | O-Glycosylation | Uniprot | |
| S309 | O-Glycosylation | Uniprot | |
| S313 | O-Glycosylation | Uniprot | |
| S316 | O-Glycosylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S383 | O-Glycosylation | Uniprot | |
| T401 | O-Glycosylation | Uniprot | |
| S435 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S446 | Phosphorylation | P78527 (PRKDC) | Uniprot |
研究背景
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Phosphorylated by PRKDC.
Nucleus.
Binds DNA as a multimer, probably a dimer. Interacts with MRTFA, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF. Forms a nuclear ternary complex with MRTFA and SCAI. Interacts with MRTFB. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 (By similarity). Interacts with SRFBP1 (By similarity). Interacts with FOXK1. Interacts with LPXN. Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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