SLC6A20 Antibody - #DF9926

Figure 2 Retinal serine is predominately obtained from the circulation (A) % serine M3 label in tissue normalized to % serine (ser) M3 label in plasma after mice were infused with [U-13C3]serine for 2 h (n = 4). (B) Abundance of serine in plasma and tissue relative to levels when serine is infused at 2 nmol/min/g mice (n = 5 for 2 nmol/min/g and n = 4 for 10 nmol/min/g groups). (C) % glycine M2 label in tissue normalized to % glycine (gly) M2 label in plasma after mice were infused with [U-13C2]glycine for 2 h (n = 4). (D) Gene expression of Slc1a4, Slc6a20a, and Slc6a20b per copy of housekeeping gene (36b4) across tissue types (n = 5). (E) Immunofluorescence visualization of SLC1A4 in the mouse retina (SLC1A4, magenta; glutamine synthetase [GS], green; DAPI, blue). ONL, outer nuclear layer; INL, inner nuclear layer; RPE, retinal pigment epithelium. White arrows indicate co-staining of Muller glia. Schematic prepared in BioRender. (F) Immunofluorescence visualization of SLC6A20 in mouse retina (SLC6A20, red; glutamine synthetase [GS], green; DAPI, blue). ONL, outer nuclear layer; INL, inner nuclear layer; RPE, retinal pigment epithelium. Data are mean ± SD (A–C) or mean ± SEM (D) and were analyzed using one-way ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.